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近年来,我国云南省及广东省海南岛均发现对氯喹有抗药性的恶性疟原虫。为调查抗性虫株的分布及其抗性程度,并寻找新药,我们采用液氮冻存法将海南岛一恶性疟原虫虫株带回实验室复苏,参照Trager及Jenaen的蜡烛缸培养法培养,用微量法以减虫率作观察指标,测定原虫对药物的敏感性。证明该株对氯喹已产生抗药性,体外培养两个月左右仍有明显抗药性,经连续转种培养3～4个月,抗性自行消失。现将结果
In recent years, chloroquine-resistant Plasmodium falciparum has been found in Yunnan Province of China and Hainan Island of Guangdong Province. To investigate the distribution and resistance level of resistant strains and to search for new drugs, we used a liquid nitrogen cryo-storage method to bring a Hainan Plasmodium falciparum strain back to laboratory for recovery following the culture of candle and cylinder cultures by Trager and Jenaen , Using microdialysis to reduce the worm rate as an indicator to determine the parasite sensitivity to drugs. The results showed that the strain was resistant to chloroquine. After cultured in vitro for about two months, it still showed obvious resistance. After continuous transplanting for 3 to 4 months, the resistance disappeared on its own. Now the result