目的:探讨菖蒲郁金汤对多发性抽动症模型大鼠行为及脑组织IL-6及TNF-α的影响。方法:82只雄性大鼠随机分为空白组12只,造模组70只,采用亚氨基二丙腈(IDPN)腹腔注射法制备多发性抽动症动物模型,造模成功后,除去体重偏差较大的10只大鼠,将造模组其余60只大鼠再次分为:模型组、泰必利组、菖蒲郁金汤高、中、低剂量组,各12只。从造模成功后第2天开始给予灌胃,泰必利组给予泰必利(47.91mg/kg),菖蒲郁金汤各剂量组给予菖蒲郁金汤(77.28g/kg、38.64g/kg、19.32g/kg),空白组与模型组给予等体积纯净水,连续给药4周。造模成功后及每周灌胃后1小时,观察大鼠行为。在最后一次给药后24小时计算胸腺指数、脾脏指数,用ELISA法检测IL-6、TNF-α水平;观察纹状体组织病理学变化。结果:1造模后,各组大鼠均出现刻板行为和运动行为;治疗4周后,泰必利组及菖蒲郁金汤组(77.28/kg、38.64g/kg)刻板行为评分明显下降;泰必利组及菖蒲郁金汤组(77.28/kg)运动行为评分明显下降。2模型组大鼠胸腺指数和脾脏指数均明显下降;菖蒲郁金汤高剂量(77.28g/kg)组可明显提高胸腺指数和脾脏指数。3各组大鼠脑组织中IL-6含量无明显变化;模型组大鼠脑组织中TNF-α水平明显下降;泰必利(47.91mg/kg)组大鼠脑组织中TNF-α水平无明显变化;菖蒲郁金汤高剂量(77.28/kg)组大鼠脑组织中TNF-α水平明显升高。4空白组大鼠纹状体神经细胞分布均匀、大小适中、形态如常;模型组大鼠纹状体神经细胞减少,以神经元细胞减少为主,且神经元细胞体积缩小,形态不规则;泰必利(47.91mg/kg)组大鼠纹状体神经细胞数量及形态变化与模型组无明显区别;菖蒲郁金汤各剂量(77.28g/kg、38.64g/kg、19.32g/kg)组纹状体神经元细胞数量及形态均有所改善,以高剂量(77.28g/kg)组为最显著。结论:菖蒲郁金汤具有抗抽动作用,其抗抽动作用可能与改善多发性抽动症模型大鼠免疫功能及修复神经细胞有关。 Objective: To investigate the effect of iris Yujin decoction on the behavior and the level of IL-6 and TNF-α in brain of rats with multiple tic disorder. Methods: Eighty - two male rats were randomly divided into blank control group (n = 12) and model group (n = 70). The animal model of multiple tic disorder was prepared by intraperitoneal injection of iminodipropionitrile (IDPN) A large group of 10 rats were divided into the model group, the tiapride group, the calamus root decoction high, medium and low dose groups, 12 rats in each group. Administration of tacrolimus (47.91 mg / kg) was given to rats from the second day after successful modeling, and irisolumin (77.28 g / kg, 38.64 g / kg, 19.32 g / kg), the blank group and model group were given equal volume of pure water, continuous administration for 4 weeks. After the success of modeling and 1 hour after intragastric administration, the behavior of rats was observed. The thymus index and spleen index were calculated 24 hours after the last administration, and the levels of IL-6 and TNF-α were detected by ELISA. The histopathological changes of striatum were observed. After 4 weeks of treatment, the stereotypic scores of tibiae and calamus root decoction groups (77.28 / kg, 38.64g / kg) were significantly decreased. The scores of stereotypic behavior and movement of rats in each group were significantly decreased The scores of exercise behavior in the Willie group and the iris Yujing decoction group (77.28 / kg) decreased significantly. 2 model group rats thymus index and spleen index were significantly decreased; high-dose calamusyujin Tang (77.28g / kg) group can significantly improve the thymus index and spleen index. The content of IL-6 in the brain tissue of rats in each group had no significant change. The level of TNF-α in the model group rats decreased significantly. The level of TNF-α in the brain of rats in the group of paclitaxel (47.91mg / kg) The level of TNF-αin the high dose (77.28 / kg) group was significantly increased. In the blank group, the number of striatum neurons in the blank group was uniform, the size was moderate, and the morphology was normal. In the model group, the number of the neurons in the striatum decreased, the number of the neurons decreased, and the neurons became smaller and irregular in shape. There was no significant difference between the model group and the number and morphology of the striatum neurons in the group of Pallion (47.91 mg / kg) The numbers and morphology of neurons in the striatum all improved, especially in the high dose group (77.28g / kg). Conclusion: Ipomoea lotus decoction has anti-tic effect and its anti-tic effect may be related to the improvement of immune function and repair of nerve cells in rats with multiple tic disorder.