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目的 从细胞水平研究异紫堇啡碱对PGF2α诱导心肌肥大的作用。方法 利用培养的新生大鼠心肌细胞 ,以细胞直径大小和蛋白含量为心肌肥大反应的指标 ,用Fura 2 /AM为荧光指示剂测定心肌细胞内钙浓度。结果 异紫堇啡碱 10 4mol/L可明显抑制PGF2α诱导的心肌细胞肥大和细胞内钙增加。结论 异紫堇啡碱可抑制PGF2α诱导心肌细胞肥大 ,该作用可能与其抑制细胞内钙增加有关
Objective To investigate the effect of indoxyphoridine on PGF2α-induced cardiac hypertrophy at the cellular level. Methods The cultured neonatal rat cardiomyocytes were used to measure the intracellular calcium concentration of cardiomyocytes using Fura 2 /AM as a fluorescent indicator with the diameter of the cells and the protein content as indicators of myocardial hypertrophy. RESULTS Insulinophoridine 10 4 mol/L could significantly inhibit PGF2α-induced cardiomyocyte hypertrophy and intracellular calcium increase. Conclusion Isoprosin can inhibit PGF2α-induced cardiomyocyte hypertrophy, which may be related to its inhibition of intracellular calcium increase.