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目的研究宁波市HIV-1感染夫妻的免疫状况和HIV毒株的亚型分布特点和流行规律。方法收集宁波市7对已经被确认为HIV-1型阳性夫妻的全血样品,对CD4+T淋巴细胞进行绝对计数,运用巢式PCR方法扩增病毒膜蛋白env基因和gag基因区并进行序列测定。应用DNASTAR软件对序列和参考序列进行比对分析,确定基因亚型。结果成功获得11条env基因序列和13条gag基因序列,确定6株为B’亚型,2株为01_AE流行重组型,2株为07_BC流行重组型,4株为08_BC流行重组型。该11例HIV-1阳性全血的CD4+T淋巴细胞绝对数最低7个/μl,最高654个/μl,平均288个/μl。结论宁波市夫妻感染HIV-1毒株存在多种亚型,大多数的感染者免疫状况低下需要抗病毒治疗,流行形势严峻,应加强对该人群免疫状况和HIV-1毒株亚型变异的监测,及时调整防治策略。
Objective To study the immune status of HIV-1 infected couples in Ningbo and the subtypes and distribution of HIV strains. Methods Seven pairs of whole blood samples of HIV-1-positive couples were collected in Ningbo. Absolute counts of CD4 + T lymphocytes were carried out. The viral envelope proteins env gene and gag gene were amplified by nested PCR and sequenced Determination. DNASTAR software was used to compare the sequences and reference sequences to determine the genotype. Results Eleven env gene sequences and 13 gag gene sequences were successfully obtained. Six isolates were identified as B ’subtypes, two were 01_AE epidemic recombinant strains, two were 07_BC epidemic recombinant strains and 4 were 08_BC epidemic recombinant strains. The absolute number of CD4 + T lymphocytes in the 11 HIV-1 positive whole blood was at least 7 / μl, up to 654 / μl with an average of 288 cells / μl. Conclusion There are many subtypes of HIV-1 strains infected by husband and wife in Ningbo City. Most of the infected patients need anti-virus treatment because of their low immune status. The epidemic situation is severe. The immune status of HIV-1 strains and the variation of subtypes of HIV-1 strains should be strengthened Monitoring, timely adjustment of prevention and control strategies.