论文部分内容阅读
Conformation of the two molecular forms, E_3 and E_4, of glueoamylase (EC 3.2.1.3.) fromMonaseus rubiginosus Sato has been studied with ultraviolet difference spectral and circulardiehroism spectral methods. The UV difference spectra produced by competitive inhibitor mai-tirol was mainly contributed by tryptophyl residue. The fraction of tryptophyl residues exposedto ethylene glycol was 0.31%, to polyethyleno glycol was 0.21% for E_3, those for E_4 were0.47% and 0.19%, respectively. The a-helix contents calculated from the far UV eireulardiehroism spectra were 22.8% in E_3 and 15.3% in E_4, which mesas that the E_3 moleculehad a more compact conformation than E_4.
Conformation of the two molecular forms, E_3 and E_4, of glueoamylase (EC 3.2.1.3.) From Monascus rubiginosus Sato has been studied with ultraviolet difference spectral and circular diehroism spectral methods. The UV difference spectra produced by competitive inhibitor mai-tirol was primarily contributed by The fraction of tryptophyl residues exposed to ethylene glycol was 0.31%, to polyethylen glycol was 0.21% for E_3, those for E_4 were 0.47% and 0.19%, respectively. The a-helix contents calculated from the far UV eireular diehism spectra were 22.8% in E_3 and 15.3% in E_4, which mesas that the E_3 moleculehad a more compact conformation than E_4.