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目的明确吸烟对慢性阻塞性肺疾病(COPD)患者外周血中Th17细胞分布的影响,探讨Th17细胞在全身炎症反应中的作用。方法使用流式细胞仪测定26例COPD患者,22例肺功能正常的吸烟者以及20例健康志愿者外周血中的Th17细胞的比例,通过回归分析Th17细胞与患者临床指标的相关性。结果 COPD患者外周血Th17细胞的比例显著高于肺功能正常的吸烟者与健康非吸烟者(2.19%、1.55%和1.16%,P<0.05)。Th17细胞的比例与1秒钟用力呼气容积(FEV1.0)占预计值的百分比呈负相关(r=-0.67,P<0.05)。结论 Th17细胞参与COPD的发病,Th17细胞的增高提示气流受限的严重程度加重。
Objective To investigate the effect of smoking on the distribution of Th17 cells in peripheral blood of patients with chronic obstructive pulmonary disease (COPD) and to explore the role of Th17 cells in the systemic inflammatory response. Methods Flow cytometry was used to determine the proportion of Th17 cells in peripheral blood of 26 patients with COPD, 22 patients with normal lung function and 20 healthy volunteers. The correlation between Th17 cells and clinical parameters was analyzed by regression. Results The proportion of Th17 cells in peripheral blood of COPD patients was significantly higher than that of smokers and healthy non-smokers with normal lung function (2.19%, 1.55% and 1.16%, P <0.05). The proportion of Th17 cells was negatively correlated with the predicted forced expiratory volume in 1 second (FEV1.0) (r = -0.67, P <0.05). Conclusions Th17 cells are involved in the pathogenesis of COPD. The increase of Th17 cells suggests that the severity of airflow limitation is aggravated.