非标记抗体酶免疫试验(PAP试验)是由Stenberger等首先提出的一种简便而实用的免疫学技术、在免疫组化研究中得到广泛地应用.由于试验的全部过程均从抗原体反应的方式进行、要求PAP复合物中的抗辣根过氧化物酶抗体(抗-HRP)具有与第一抗体相同的种系特异性、因而使PAP复合在应用上受到其中抗-HRP种系来源的限制 The unlabeled antibody enzyme immunoassay (PAP test) is a simple and practical immunological technique first proposed by Stenberger et al., Which is widely used in immunohistochemistry research. Since the whole process of the test is based on the way of antigen reaction , Requires that the anti-horseradish peroxidase antibody (anti-HRP) in the PAP complex have the same germline specificity as the primary antibody, thus rendering PAP recombination impractical for applications where the origin of the anti-HRP germline is limited