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本文提出了流动注射络合荧光法同时测定微量强力霉素和维生素B_2新方法。在中性条件下,维生素B_2在汞灯激发下于520hm发射荧光,而强力霉素与0.10mol/L MgSO_4溶液快速反应生成的荧光络合物也在520nm发射荧光,它们的荧光强度在维生素B_2和强力霉素浓度比为10∶1～1∶25范围内有加合性,由此同时测定了混合物中的维生素B_2和强力霉素。本法测定尿液中维生素B_2和强力霉素的回收率为95%～102％。
In this paper, a new method for the simultaneous determination of trace amounts of doxycycline and vitamin B 2 by flow injection complexing fluorescence was proposed. Under neutral condition, vitamin B 2 emits fluorescence at 520 nm under the excitation of mercury lamp, while the fluorescent complex formed by the rapid reaction of doxycycline and 0.10 mol / L MgSO 4 solution also emits fluorescence at 520 nm. The fluorescence intensity of vitamin B 2 in vitamin B 2 And doxycycline concentration ratio of 10: 1 to 1:25 within the adductivity, thus simultaneously measuring the mixture of vitamin B_2 and doxycycline. The determination of urine vitamin D 2 and doxycycline recovery of 95% to 102%.