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用含ＨＢＶ全基因和ＨＢＶ大、中、小分子表面蛋白基因的真核细胞表达质粒（ＣＭＶ－ＨＢＶ、ＣＭＶ－ＬＳ、ＣＭＶ－ＭＳ、ＣＭＶ－Ｓ）分别与含ＨＤＶ ｃＤＮＡ三聚体的重组质粒（ｐＳＶＬＤ３）共转染ＣＨＯ细胞。转染后３天在上述４种转染细胞内及培养上清中均检出了ＨＤＶＲＮＡ和ＨＤＡｇ，表明上述４种转染细胞的培养上清中均有ＨＤＶ病毒颗粒的包装和分泌。提示ＨＤＶ病毒的包装可能仅需ＨＢＶＳ基因及其小分子表面蛋白的辅助。
The eukaryotic expression plasmids (CMV-HBV, CMV-LS, CMV-MS, CMV-S) containing HBV full-length gene and HBV large, medium and small molecule surface protein genes were respectively used in combination with the recombinant plasmids containing the HDV cDNA trimer (PSVLD3) were co-transfected into CHO cells. Three days after transfection, HDVRNA and HDAg were detected in the four kinds of transfected cells and in the culture supernatant, indicating that HDV virus particles were both packed and secreted in the culture supernatants of the four transfected cells. Suggesting that HDV virus packaging may only require the help of the HBVS gene and its small molecule surface proteins.