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目的建立兔急性视网膜坏死模型并探讨该病对侧眼发病的感染途径。设计实验性研究。研究对象41只成年青紫蓝兔。方法经睫状体扁平部将单纯疱疹病毒(HSV-1)注射于兔视乳头旁的视网膜下,建立急性视网膜坏死(ARN)动物模型。在注射病毒后1、3天开始对模型动物分别用更昔洛韦玻璃体腔注射、静脉注射以及玻璃体腔注射联合静脉注射三种方式治疗。通过PCR方法检测血液中是否存在病毒。部分动物做眼球和脑组织病理检查。主要指标血液的PCR病毒学检测结果,眼球、视神经、脑组织的病理改变。结果10只兔在接种病毒后10～18天双眼先后发生ARN,15只兔出现神经系统损害。双眼发病的动物中,5只兔在对侧眼发病前后(病毒注射后9～19天),血液PCR中均未检测到病毒存在。眼球、视神经、脑组织均有淋巴细胞和浆细胞浸润。结论可于兔视网膜下注射HSV-1建立ARN模型。在兔ARN模型中双眼病变者未发现病毒通过血液感染的征象,是否经视神经途径感染有待进一步研究。
Objective To establish a rabbit model of acute retinal necrosis and investigate the pathogenic mechanism of the disease on the lateral eye. Design experimental research. Subjects 41 adult blue purple blue rabbit. Methods Herpes simplex virus (HSV-1) was injected into the retina next to the retina via the ciliary body to establish an acute retinal necrosis (ARN) animal model. The model animals were treated with ganciclovir vitreous cavity injection, intravenous injection and intravitreal injection combined with intravenous injection three days after the virus was injected. The presence or absence of virus in the blood is detected by the PCR method. Some animals do eye and brain histopathology. The main indicators of blood PCR virology test results, eye, optic nerve, pathological changes of brain tissue. Results Ten rabbits were found to have ARN in both eyes 10 to 18 days after inoculation with virus, and nervous system damage occurred in 15 rabbits. Of the animals with binocular disease, no virus was detected in 5 of the 5 rabbits before and after contralateral eye onset (9 to 19 days after virus injection) and blood PCR. Eye, optic nerve, brain tissue lymphocytes and plasma cells infiltration. Conclusion HSV-1 can be injected subretinally in rabbits to establish ARN model. In rabbits ARN model binocular lesions were found no signs of the virus through the bloodstream infection, whether the optic nerve pathway infection for further study.