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目的 :探讨CpGODN对老年小鼠的体液和细胞免疫应答的增强作用。方法 :选用老年C5 7BL 6小鼠 ,将乙肝疫苗和 10 μg、2 0 μgCpGODN同时或单独肌注到小鼠体内 ,两周后以同样剂量加强免疫一次 ,再过 3周后摘除眼球取血 ,用ELISA方法检测抗HBsIgG抗体和IL 12 ;无菌取脾脏作HE染色 ,观察脾脏淋巴细胞变化。结果 :10 μg和 2 0 μgCpGODN与疫苗同时注射组产生的抗体绝对量分别是单独注射疫苗组的 3倍和 4倍 ;产生的IL 12水平较对照组有明显升高 ,且 2 0 μg比 10μgCpG组产生的IL 12水平更高。光镜下各组的脾脏淋巴细胞的变化如下 :正常老年鼠组脾脏淋巴细胞较正常青年鼠组明显稀少 ;老年鼠 + 10 μgCpG组脾脏淋巴细胞较正常老年鼠组有了明显增加 ,且细胞核也明显增大 ;2 0 μgCpG组增加的更加明显。结论 :CpGODN能增强乙肝疫苗在老年小鼠中的体液和细胞免疫应答。
Objective: To investigate the potentiation effect of CpGODN on humoral and cellular immune responses in aged mice. Methods: Aged mice were injected intraperitoneally with 10 μg or 20 μg CpG ODN into mice respectively. Two weeks later, the mice were boosted with the same dose once more. After 3 weeks, The anti-HBs IgG antibody and IL 12 were detected by ELISA. The spleen was taken out by HE staining and the spleen lymphocytes were observed. Results: The absolute amount of antibody produced by simultaneous injection of 10 μg and 20 μg CpG ODN and the vaccine was 3 and 4 times higher than that of the vaccine alone. The level of IL 12 produced was significantly higher than that of the control group, and 20 μg was more than 10 μg CpG The group produced higher levels of IL 12. The changes of spleen lymphocytes in each group under light microscope were as follows: the spleen lymphocytes of normal aged mice group were obviously less than that of normal young mice group; the spleen lymphocytes of aged mice + 10 μgCpG group had a significant increase compared with normal old mice group, Significantly increased; 2 0 μgCpG group increased more pronounced. Conclusion: CpGODN can enhance the humoral and cellular immune responses of hepatitis B vaccine in aged mice.