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目的了解早期应用冬虫夏草提取液(CSAE)治疗对病毒性心肌炎(VMC)小鼠心肌病变、血清IFN-γ水平及脾脏T细胞亚群的影响。方法100只成年雄性BALB/c小鼠随机分为正常对照组(CG)、感染组(IG)和CSAE治疗组(CTG)。IG及CTG小鼠腹腔感染柯萨奇病毒B3(CVB3),于感染CVB3后第7天和第14天,计算小鼠的生存率,然后并分批处死,观察心肌组织的病理变化及用ELISA法检测血清IFN-γ的水平。用流式细胞术分析脾脏中CD3+、CD4+、CD8+T细胞的百分率。结果与CG组相比较,IG小鼠血清IFN-γ的水平及脾脏中各T细胞亚群的百分率均降低;而CD4+/CD8+T细胞的比例升高。CTG小鼠的心肌炎症坏死较轻,感染病毒后14d的存活率为85%,显著高于IG的55%(P<0.05)。血清IFN-γ的水平及脾脏中CD3+、CD8+T细胞的百分率显著高于IG组。CTG小鼠脾脏中各T细胞亚群的百分率及CD4+/CD8+T细胞的比例与CG组无显著差异。结论CSAE可诱导VMC小鼠IFN-γ产生并调节细胞免疫功能,对VMC有一定的治疗作用。
Objective To investigate the effects of early application of Cordyceps sinensis extract (CSAE) on myocardial lesions, serum IFN-γ levels and splenic T cell subsets in viral myocarditis (VMC) mice. Methods One hundred adult male BALB/c mice were randomly divided into normal control group (CG), infection group (IG) and CSAE treatment group (CTG). IG and CTG mice were infected intraperitoneally with Coxsackievirus B3 (CVB3). After 7 days and 14 days after infection with CVB3, the survival rate of mice was calculated. Then they were sacrificed in batches to observe the pathological changes of myocardial tissue and ELISA. The serum IFN-γ levels were measured. The percentage of CD3+, CD4+, and CD8+ T cells in the spleen was analyzed by flow cytometry. Results Compared with the CG group, the levels of serum IFN-γ in IG mice and the percentage of T cell subpopulations in the spleen were all decreased, while the proportion of CD4+/CD8+ T cells was increased. In CTG mice, the inflammation and necrosis of myocardium were lighter. The survival rate at 14 days after infection was 85%, which was significantly higher than that of IG (55%) (P<0.05). The level of serum IFN-γ and the percentage of CD3+ and CD8+ T cells in the spleen were significantly higher than those in the IG group. The percentage of T cell subsets and the proportion of CD4+/CD8+ T cells in the spleen of CTG mice were not significantly different from those in the CG group. Conclusion CSAE can induce IFN-γ production in VMC mice and regulate cellular immune function. It has a certain therapeutic effect on VMC.