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目的探讨脑积水对大鼠脑室周围神经干细胞影响。方法 6 w Wistar大鼠70只,随机分为实验组35只,对照组35只,应用显微外科技术向枕大池内注入25%无菌高岭土混悬液0.05 ml,分别在高岭土注射后3 d,1 w,2 w,3 w,4 w处死动物,迅速取脑组织,测侧脑室指数,用免疫组织化学方法动态检测脑室周围Nestin阳性细胞的表达,同样方法向枕大池内注入生理盐水作为对照组。结果实验组28只大鼠成功诱发脑积水并完成实验全过程,对照组30只完成实验全过程。对照组大鼠各对应时间点,侧脑室指数基本相同,实验组大鼠脑室进行性扩大;脑室周围Nestin阳性细胞:对照组细胞数平均193±20.3个并维持,脑积水造模后3 d达到最大值,为对照组308%±1%(P<0.001),1 w脑室周围Nestin阳性细胞下降到对照组196%±1%,2 w降到对照组水平210±22.4个,3 w脑室周围Nestin阳性细胞几乎看不到并持续。结论脑积水引起脑室进行性扩大,脑室周围神经干细胞可能受脑室扩张机械性压迫引起缺血,缺氧影响,参与脑积水病理损害和修复过程。
Objective To investigate the effects of hydrocephalus on neural stem cells in periventricular cells of rats. Methods Sixty Wistar rats were randomly divided into experimental group (n = 35) and control group (n = 35). Microsurgical techniques were used to inject 0.05% sterile kaolin suspension into the occipital cistern. After 3 days of kaolin injection, Animals were sacrificed at 1 w, 2 w, 3 w, and 4 w. Brain tissue was taken immediately and index of lateral ventricle was measured. The expression of Nestin positive cells in periventricular was detected by immunohistochemical method. In the same way, physiological saline Control group. Results 28 rats in the experimental group were successfully induced hydrocephalus and completed the whole process of the experiment. The control group of 30 rats completed the whole experiment. The control group rats at each corresponding time point, lateral ventricle index was basically the same, experimental group rats ventricular enlargement; Nestin positive cells around the ventricle: the control group average number of cells 193 ± 20.3 and maintenance, hydrocephalus modeling 3 d (P <0.001). The number of Nestin positive cells in the periventricular zone decreased to 196% ± 1% in the control group at 1 w and 210 ± 22.4 at 2 w in the control group (P <0.001) Nestin-positive cells around can hardly be seen and continue. Conclusions Hydrocephalus causes ventricular enlargement. Peripheral nerve stem cells may be impaired by ischemia and hypoxia due to mechanical compression of ventricular dilatation. It may participate in pathological damage and repair of hydrocephalus.