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目的对吉林松原郭尔罗斯自治县蒙古族和汉族人群中2型糖尿病(T2MD)患者CD36内含子3(intron 3)[TG]重复序列基因多态性分布情况进行研究,探讨该基因多态性与2型糖尿病的相关性。方法应用PCR和直接测序法对40例正常组和193例2型糖尿病患者CD36内含子3[TG]重复序列基因多态性进行分析。结果①CD36内含子3[TG]重复序列有5种单体型,即[TG]重复次数为11、12、13、15、16次。②正常组与2型糖尿病患者之间CD36内含子3[TG]重复序列各种单体型及基因型分布差异无统计学意义(P>0.05)。③蒙古族与汉族之间CD36内含子3[TG]重复序列各种单体型及基因型分布差异无统计学意义(P>0.05)。④CD36内含子3[TG]重复序列13次的单体型及基因型在蒙古族人群中正常组与2型糖尿病患者之间分布差异具有统计学意义(P<0.05)。结论 CD36内含子3[TG]重复次数13次的单体型是松原蒙古族发生2型糖尿病的危险基因。
Objective To investigate the distribution of CD36 intron 3 (TG) repeat polymorphism in patients with type 2 diabetes mellitus (T2MD) in Mongolian and Han nationalities in Songliao Guorrosi Autonomous County of Jilin Province, and to explore the relationship between the gene polymorphism Correlation with type 2 diabetes. Methods The polymorphisms of CD36 intron 3 (TG) repeats in 40 normal controls and 193 patients with type 2 diabetes mellitus were analyzed by PCR and direct sequencing. Results ① There were 5 haplotypes in CD36 intron 3 [TG] repeats, that is, the number of [TG] repeats was 11, 12, 13, 15 and 16 times. ② The distribution of haplotypes and genotypes of CD36 intron 3 [TG] repeats between normal group and type 2 diabetic patients was not statistically different (P> 0.05). ③ There was no significant difference in the distribution of haplotypes and genotypes among CD36 intron 3 [TG] repeats between Mongolian and Han nationality (P> 0.05). ④ The haplotypes and genotypes of 13 repeats of CD36 intron 3 [TG] were significantly different between Mongolian subjects and type 2 diabetic patients (P <0.05). Conclusion The haplotype of 13 repeats of CD36 intron 3 [TG] is the risk gene of type 2 diabetes in Songyuan Mongolian.