目的建立龙血竭中5种有效成分的一测多评法,探讨一测多评法在龙血竭质量控制中的应用。方法采用高效液相色谱法,使用Fortis Xi C18(250 mm×4.6 mm,5μm)色谱柱,流动相为乙腈(A)-1.0%冰醋酸水溶液(B),梯度洗脱,0~10 min,25%~30%A;10~60 min,30%~50%A,体积流量1.0 mL/min;检测波长278 nm;柱温30℃;进样量10μL。以紫檀茋为内参,分别建立7,4′-二羟基黄酮、白藜芦醇、龙血素A和龙血素B相对于紫檀茋的相对校正因子,分别采用外标法和一测多评法测定5种成分的量,并比较二者结果的相对误差。结果 7,4′-二羟基黄酮质量浓度在10.23~102.27μg/mL、白藜芦醇质量浓度在11.01~110.14μg/mL、龙血素A质量浓度在9.47~94.72μg/mL、龙血素B质量浓度在11.59~115.90μg/mL、紫檀茋质量浓度在24.35~243.52μg/mL线性关系良好;4种化学成分相对于紫檀茋的相对校正因子分别为0.626、1.064、1.154、0.837;且在不同实验条件下耐用性良好(RSD<3.0%);一测多评法的计算结果与外标法测得结果无显著差异。结论建立的一测多评法可作为龙血竭中5种有效成分的测定方法,一测多评法为龙血竭质量控制提供了新的模式与方法。 OBJECTIVE To establish a multi-assessment method for evaluating the five active ingredients in DRG, and to explore the application of multi-assessment method in DRG quality control. Methods High performance liquid chromatography (HPLC) was performed on a Fortis Xi C18 (250 mm × 4.6 mm, 5 μm) column with a mobile phase of acetonitrile (A) -1.0% glacial acetic acid (B) 25% -30% A; 10-60 min, 30% -50% A, volume flow 1.0 mL / min; detection wavelength 278 nm; column temperature 30 ℃; Taking the red sandalwood as the internal reference, the relative correction factors of 7,4’-dihydroxyflavone, resveratrol, loureirin A and loureirin B were compared with those of the sandalwood Method to determine the amount of five components, and compare the relative error between the two results. Results The mass concentration of 7,4’-dihydroxyflavone was 10.23 ~ 102.27μg / mL, the concentration of resveratrol was 11.01 ~ 110.14μg / mL, the concentration of hematin A was 9.47 ~ 94.72μg / mL, The linear calibration curves for the concentrations of B, B and C were 11.59 ~ 115.90μg / mL and 23.35 ~ 243.52μg / mL, respectively. The relative calibration factors of the four chemical constituents were 0.626, 1.064, 11.54 and 0.837, Under different experimental conditions, the durability was good (RSD <3.0%). There was no significant difference between the calculated results from one test and those from external standard test. Conclusion The multi-evaluation method established by this method can be used as a method to determine the five active ingredients in DRG. A multi-evaluation method provides a new model and method for DRG quality control.