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目的探讨高渗盐水预处理对大鼠肝脏缺血再灌注损伤一氧化氮(NO)、内皮素-1(ET-1)表达水平的影响及其相关性分析。方法清洁健康雄性SD大鼠45只,随机分为假手术组、肝脏缺血再灌注组和高渗盐水预处理组3组,每组15只。用Pringle’s法建立大鼠肝脏缺血再灌注模型,检测肝脏缺血30 min再灌注1、6、24 h后血清NO、ET-1的水平,探讨缺血再灌注6 h时的NO、ET-1水平的相关性。结果肝脏缺血30 min再灌注1、6、24 h后,缺血再灌注组和高渗盐水预处理组血清NO水平均明显低于假手术组(P<0.01),而血清ET-1水平则均明显高于假手术组(P<0.01);高渗盐水预处理组血清NO水平明显高于缺血再灌注组(P<0.01),而血清ET-1水平则明显低于缺血再灌注组(P<0.01)。各组血清NO和ET-1水平在1、6、24 h之间的变化以6 h最为显著。大鼠血清NO与ET-1水平呈负相关(r=-0.970,P<0.01)。结论高渗盐水预处理改变了缺血再灌注损伤后血清NO与ET-1的水平,且二者水平的变化具有相关性,其作用机理可能是通过某种途径激发血清NO的水平、降低ET-1的水平,使两者的动态平衡向良性方向发展而达到保护作用。
Objective To investigate the effects of hypertonic saline preconditioning on the expression of nitric oxide (NO) and endothelin-1 (ET-1) after hepatic ischemia-reperfusion injury in rats and its correlation analysis. Methods Forty-five healthy male Sprague-Dawley rats were randomly divided into sham-operated group, hepatic ischemia-reperfusion group and hypertonic saline pretreatment group, 15 rats in each group. Pringle’s method was used to establish the rat model of hepatic ischemia-reperfusion. The levels of NO and ET-1 in serum were detected at 1, 6 and 24 h after ischemia reperfusion for 30 min, and the levels of NO and ET- 1 level of relevance. Results Serum NO levels in ischemic reperfusion group and hypertonic saline preconditioning group were significantly lower than those in sham operation group (P <0.01) at 30 min after reperfusion at 30 min after reperfusion, while serum ET-1 level (P <0.01). The level of serum NO in hypertonic saline pretreatment group was significantly higher than that of ischemia reperfusion group (P <0.01), but the level of ET-1 in serum was significantly lower than that in ischemia group Perfusion group (P <0.01). The change of serum NO and ET-1 levels in each group between 1, 6 and 24 h was the most significant at 6 h. Serum NO was negatively correlated with ET-1 level (r = -0.970, P <0.01). CONCLUSION: Hypertonic saline pretreatment can change the level of serum NO and ET-1 after ischemia-reperfusion injury, and the changes of serum NO and ET-1 levels are correlated. The mechanism of hypertonic saline preconditioning may be to stimulate serum NO level by some means and decrease ET -1 level, so that the dynamic balance between the two directions to the development of a protective effect.