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单孢子分离是纯化菌种、复壮菌株必不可少的手段。本人在长期的单孢子分离中不断摸索,总结出一个比往常用方法更为简便的分离方法。该方法克服了以往方法首先都必须稀释成悬浮液,然后滴到培养皿中,再用解剖镜慢慢寻找只有单个孢子的水滴等缺陷;可直接将已产生孢子的斜面或平面菌落的菌丝挑入倒有琼胶培养基的培养皿上,置于解剖镜下,找到这一块菌丝;再用挑针将单个孢子挑(扒)到培养皿另外洁净的地方,然后用移植器切下这块只有单个孢子的琼胶培养基,移入斜面试管即可。该方法既快又简单,污染率只在5％以下。现介绍如下:
Isolation of monospores is an indispensable means of purifying strains and rejuvenating strains. I am constantly groping in the long-term single spore separation, summarizing a more simple method of separation than usual. The method overcomes the previous method must first be diluted into a suspension, and then dripped into the Petri dish, and then slowly using a dissecting microscope to find only a single spore of water droplets and other defects; can have been spores generated ramp or plane colonies mycelium Pick up the agar plate with the agar medium and place it under the microscope to find the piece of mycelium. Then pick the individual spores with a pick-up needle to a clean place in the dish and cut it with a transplanter This piece of agar medium with only a single spore can be moved into a beveled tube. The method is fast and easy, with pollution rates below 5%. Now introduced as follows: