论文部分内容阅读
目的 :研究胶质纤维酸性蛋白 ( GFAP)和 S-10 0蛋白 ( S-10 0 )免疫组化染色在颅内肿瘤中的诊断价值。方法 :对 14 8例颅内肿瘤的手术切除标本进行常规外检取材 ,福尔马林固定 ,石蜡包埋 ,切片厚 5 μm,HE染色 ;对其连续切片用 GFAP和 S-10 0抗体进行免疫组化染色 ,跟踪随访 5 5例胶质瘤患者。结果 :GFAP存在于各级星形胶质细胞瘤、室管膜瘤、多形性胶质母细胞瘤、少枝胶质细胞瘤、混合性胶质瘤的星形细胞成份。S-10 0分布于颅内胶质来源的和非胶质来源的肿瘤。随着胶质瘤恶性程度的增加 ,GFAP免疫染色逐渐减弱 ( P<0 .0 0 1) ,2年生存率越低 ,预后越差 ( P<0 .0 0 1)。结论 :GFAP和 S-10 0有助于颅内肿瘤的诊断和鉴别诊断 ,GFAP表达的检测可作为胶质源性肿瘤的确定和预后评估的重要参考指标
Objective: To investigate the diagnostic value of immunohistochemical staining of glial fibrillary acidic protein (GFAP) and S-10 protein (S-10 0) in intracranial tumors. Methods: Forty-eight cases of intracranial tumors were routinely excised, formalin-fixed and paraffin-embedded. The sections were stained with 5 μm sections and stained with HE. The sections were stained with GFAP and S-10 0 antibody Immunohistochemical staining was followed up in 55 patients with glioma. RESULTS: GFAP was found in astrocytoma of astrocytoma, ependymoma, glioblastoma multiforme, glioblastoma multiforme and mixed glioma. S-10 0 Distribution of intracranial glial derived and non-glial derived tumors. With the increase of the malignant degree of glioma, the immunostaining of GFAP gradually decreased (P <0.01). The lower the 2-year survival rate was, the worse the prognosis was (P <0.01). CONCLUSION: GFAP and S-10 0 are helpful for the diagnosis and differential diagnosis of intracranial tumors. The detection of GFAP expression can be used as an important reference for the determination and prognosis evaluation of glioma-derived tumors