银耳及香灰菌为互生菌。香灰菌将木材中不易被银耳利用的物质分解,为银耳的生育提供良好的营养条件。一般银耳菌丝的分离是采用耳木分离法,分离到的菌种难以确保为纯银耳菌丝,是非银耳及耳友的混合菌丝。我们采用原生质体再生技术进一步分离,得到由单个银耳及香灰菌原生质体萌发形成的纯菌丝。 Tremella and fragrant gray bacteria for each other bacteria. Gray scent of wood in the material is not easy to be used Tremella decomposed for the growth of Tremella good nutrition. General fungus mycelium separation is the use of ear wood separation method, isolated strains difficult to ensure pure fungus mycelium, non-white fungus and ear of a mixed mycelium. We use protoplast regeneration technology to further separation, obtained by a single white fungus and gray mold germination of protoplasts formed pure mycelium.