论文部分内容阅读
植物名称菘蓝(Isatis indigotica) 材料菘蓝幼苗叶片。培养条件愈伤组织诱导培养基为MS_+NAA1mg/l(单位下同)+BA0.1～0.2,生根培养基为MS+NAA1～2。25±1℃下培养,每天光照10小时,光强1500lx。结果和讨论经1周培养后,外植体接触培养基的部分形成多少不一的绿色小颗粒。这些小颗粒再经1周左右培养发育成绿色芽丛,长约0.2～0.5cm。将诱导出芽丛的外植体转
Plant name Isatis indigotica Material Blueberry seedling leaves. Culture conditions Callus induction medium MS_ + NAA1mg / l (the same below) + BA0.1 ~ 0.2, rooting medium MS + NAA1 ~ 2.25 ± 1 ℃ culture, light for 10 hours a day, the light intensity 1500lx. RESULTS AND DISCUSSION After a week of incubation, the explants exposed to the media formed parts of green microparticles of varying sizes. These small particles and then developed by a week or so into green buds, about 0.2 ~ 0.5cm. The explants of the bud clusters will be induced to turn