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采用表面增强拉曼光谱技术结合化学计量方法快速分析脐橙果皮中的三唑磷农药残留。利用乙腈快速提取脐橙果皮的提取液,采用石墨化碳、C18和PSA去除果皮提取液中色素等物质的干扰。以脐橙果皮提取液为基质,配制不同浓度的三唑磷农药溶液,进行拉曼信号采集,得到的拉曼特征峰与三唑磷标准溶液的特征峰基本一致。建立脐橙果皮中三唑磷农药残留的偏最小二乘法预测模型,结果表明,以脐橙果皮提取液为基质的三唑磷溶液最低检测浓度为0.8 mg/L;模型对预测集样本的预测均方根误差为1.60 mg/L,相关系数为0.9669。再利用该方法检测脐橙中实际所含三唑磷农药残留,其模型对样本的预测值与真实值的相对误差绝对值在0.08%~5.98%之间,预测回收率为94.36%~104.36%,模型预测能力和重现性良好。
Rapid Determination of Triazophos Pesticide Residues in Peel of Navel Orange by Surface-Enhanced Raman Spectroscopy and Chemometric Methods. The extract of navel orange peel was extracted rapidly by using acetonitrile, and the interference of pigment and other substances in peel extract was removed by using graphitized carbon, C18 and PSA. Taking the extract of navel orange peel as the substrate, different concentrations of triazophos pesticide solution were prepared and the Raman signal was collected. The Raman peak was basically the same as that of the triazophos standard solution. The partial least square prediction model of triazophos pesticide residue in navel orange peel was established. The results showed that the lowest concentration of triazophos solution was 0.8 mg / L. The root-mean-square error was 1.60 mg / L with a correlation coefficient of 0.9669. Then the method was used to detect the residual triazophos pesticide residues in navel orange. The absolute value of the relative error between the predicted value and the true value of the model was between 0.08% and 5.98%. The predicted recovery rate was 94.36% -104.36% Model predictability and reproducibility are good.