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了解噪声暴露后耳蜗微血管内白细胞是否参与微循环障碍的形成。用 16只豚鼠暴露 115dB(SPL)的 4kHz窄带噪声 4h ,2天或 4天后处死。耳蜗行 :①台盼蓝染色 ,标记血管内失活的白细胞 ;②Hoechst33342荧光染色标记毛细胞核。部分动物处死前行静脉注射FITC 右旋糖酐 ,标记血浆 ,处死后观察血管充盈状态。结果 :耳蜗基底膜微血管发现失活的白细胞 ,嵌顿于血管内或分布于血管周围 ,造成红细胞聚集 ,管径增宽。FITC 右旋糖酐标记的血浆未出现在白细胞嵌顿的血管内 ,表明血流被阻断。白细胞嵌顿与毛细胞损伤虽都主要出现在耳蜗一回和二回 ,但二者并不一定发生在同一解剖部位。有趣的是白细胞嵌顿只出现在基底膜微血管 ,而在血管纹微血管内无此病变。提示噪声后白细胞参与耳蜗微循环障碍的形成 ,它对噪声引起的损伤和修复过程的其它影响值得进一步的研究。
To understand whether noise-induced cochlear microvascular white blood cells are involved in the formation of microcirculation. 16 guinea pigs were exposed to 4 dB narrow band noise of 115 dB (SPL) for 4 h and sacrificed after 2 or 4 days. Cochlea: ① trypan blue staining, marking the in-blood vessel inactivation of leukocytes; ② Hoechst33342 fluorescent staining of hair cell nuclei. Some animals were sacrificed before intravenous FITC dextran injection, labeled plasma, after sacrifice to observe the state of vascular filling. Results: Inactivated leukocytes were found in the basement membrane of the cochlea, implanted in the blood vessels or distributed around the blood vessels, resulting in the accumulation of red blood cells and broadening of the diameter. FITC dextran-labeled plasma did not show up in leucocyte-implanted vessels, indicating that blood flow was blocked. Although white blood cell incarceration and hair cell damage are mainly occurred in the first and second round of the cochlea, but the two do not necessarily occur in the same anatomical site. Interestingly, leukocyte incarceration only occurs in the basement membrane capillaries, but not in vascular capillaries. Prompted noise after leukocytes involved in the formation of cochlear microcirculation, its noise-induced damage and other effects of the repair process deserve further study.