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AIM: To develop and validate a liquid chromatography-tandem mass spectrometric method (LC-MS/MS) for quantifying olmesartan in human plasma. METHODS: Telmisartan was added as the internal standard,the plasma sample was extracted using acetonitrile and then separated on a Waters Xterra MS C18(2.1 mm×50 mm,5 μm)column and the column temperature was set at 40 ℃. The mobile phase consisted of acetonitrile-0.1% formic acid water solution(50∶50,v/v),the flow rate was 0.3 mL/min. The ionization was optimized using ESI (+) and enhanced selectivity was achieved using tandem mass spectrometric analysis via MRM functions, the ion of m/z 448.27-429.7 and m/z 516.43-497.8, 276.5 was used to qualify olmesartan and telmisartan, respectively. RESULTS: The concentration range of olmesartan was 2.25-2280 ng/mL, with a good linearity. The lower limit of quantitation (LLOQ) of olmesartan in plasma was 2.250 ng/mL. The extracted recovery was >60%, the relative recovery was 99%-105%. The intra-and inter-day RSD were <15%. CONCLUSION: It can be used for determination of olmesartan plasma concentration and pharmacokinetic study as a sensitive, simple and accurate method.
AIM: To develop and validate a liquid chromatography-tandem mass spectrometric method (LC-MS / MS) for quantifying olmesartan in human plasma. METHODS: Telmisartan was added as the internal standard, the plasma sample was extracted using acetonitrile and then separated on a The mobile phase consisted of acetonitrile-0.1% formic acid water solution (50:50, v / v), the flow rate was 0.3 mL / min. The ionization was optimized using ESI (+) and enhanced selectivity was achieved using tandem mass spectrometric analysis via MRM functions, the ion of m / z 448.27-429.7 and m / z 516.43-497.8, 276.5 was used The qualifier olmesartan and telmisartan, respectively. RESULTS: The concentration range of olmesartan was 2.25-2280 ng / mL, with a good linearity. The lower limit of quantitation (LLOQ) of olmesartan in plasma was 2.250 ng / mL. The extracted recovery was > 60%, the relative recovery was 99% -105%. The intra-and int er-day RSD were <15% CONCLUSION: It can be used for determination of olmesartan plasma concentration and pharmacokinetic study as a sensitive, simple and accurate method.