目的在临床病理结果报告中,往往出现免疫组化染色结果与会诊单位的染色结果不一致,导致诊断上产生分歧。本研究对免疫组化抗体以及染色操作等注意事项进行探讨,分析存在的问题。方法对科室20种常用、两个不同克隆号的抗体分为a、b两组。实验分4组进行对比实验,A组(a组抗体在link48);B组(a组抗体在Bench Mark XT);C组(b组抗体在link48);D组(b组抗体在Bench Mark XT)。结果A、B两组与标准片结果相符率为40%,C组染色结果相符率为75%,D组染色结果相符率为65%。a组抗体在两种机型的染色结果相符率为25%,b组抗体的相符率为60%。a、b组抗体分别在两种染色仪的染色结果对比有明显差异(30例,占37.5%)。显示b组抗体比a组的抗体敏感性及特异性更高,结果更可靠稳定。link48染色仪染色结果符合率更高。结论抗体及机型是否相配都会对染色结果产生影响,因此应该要重视彼此间的关系,提高染色的质量。 Purpose In clinicopathological results report, immunohistochemical staining results often inconsistent with the staining results of the consultation unit, resulting in the diagnosis of differences. In this study, immunohistochemical antibodies and staining and other precautions to be discussed, analysis of existing problems. Methods 20 kinds of commonly used departments, two different clone antibody is divided into a, b two groups. The experiment was divided into 4 groups to conduct the comparative experiment. Group A (group a antibody was on link48); group B (group A antibody on Bench Mark XT); group C (group b antibody on link48); group D (group b antibody on Bench Mark XT ). Results The coincidence rates of A and B with the standard tablets were 40%, those of C group were 75%, and those of D group were 65%. Group a antibody staining results in both models was 25%, b group antibody conformance rate of 60%. There was a significant difference in the staining results of a and b antibodies between the two staining instruments (30 cases, 37.5%). The antibody of group b showed higher antibody sensitivity and specificity than group a, and the result was more reliable and stable. link48 staining instrument staining results in line with higher rates. Conclusion Whether the antibody and the model are compatible will affect the staining results, so we should pay attention to the relationship between each other to improve the quality of staining.