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目的观察党参总皂苷(TSC)对大鼠局灶性脑缺血性损伤的保护作用,并初探其机制。方法 54只SD大鼠随机分为假手术组、假手术给药组、模型组、治疗组。假手术给药组及治疗组每日1次TSC60mg·kg~(-1)灌胃,假手术组及模型组灌胃等体积的生理盐水,7d后建立大鼠右侧大脑中动脉阻塞(MCAO)的局灶性脑缺血模型,24h后观察各组大鼠死亡率、神经功能症状,2,3,5-三苯基氯化四氮唑(TTC)染色测量脑梗死体积,real time RT-PCR法测定缺血周边区肿瘤生长因子α(Tnf-α)、白细胞介素1β(Il-1β)、环氧酶2(Cox-2)、内皮型一氧化氨合酶(Nos3)及诱导型一氧化氮合酶(Nos2)的mRNA表达。结果 TSC明显降低MCAO所致的死亡率及神经功能评分,并减少脑梗死体积(均P<0.05)。同时,TSC明显抑制了缺血周边区Tnf-α、Il-1β、Cox-2、Nos3及Nos2的mRNA表达(P<0.05),而对假手术给药组大鼠无明显影响(P>0.05)。结论 TSC对大鼠局灶性脑缺血性损伤有保护作用,其机制与抑制炎症相关基因的mRNA表达有关。
Objective To observe the protective effect of Codonopsis pilosula (TSC) on focal cerebral ischemic injury in rats and its mechanism. Methods 54 SD rats were randomly divided into sham operation group, sham operation group, model group and treatment group. The rats in the sham operation group and the treatment group were intragastrically administrated with TSC 60 mg · kg -1 once a day for 6 weeks, and the rats in the sham operation group and model group were given the same volume of normal saline. After 7 days, the right middle cerebral artery occlusion (MCAO ) After focal cerebral ischemia. After 24 hours, the mortality and neurological deficit in each group were observed. The volume of cerebral infarction was measured by 2,3,5-triphenyltetrazolium chloride (TTC) staining. Real time RT The expressions of Tnf-α, IL-1β, Cox-2, Type 2 Nitric Oxide Synthase (Nos2) mRNA Expression. Results TSC significantly reduced the mortality and neurological deficits caused by MCAO and decreased the volume of cerebral infarction (all P <0.05). At the same time, TSC significantly inhibited the mRNA expression of Tnf-α, Il-1β, Cox-2, Nos3 and Nos2 in the ischemic peripheral area (P <0.05) ). Conclusion TSC has a protective effect on focal cerebral ischemic injury in rats. Its mechanism is related to inhibiting the mRNA expression of inflammation related genes.