论文部分内容阅读
目的了解糖基化终末产物(AGES)对人内皮细胞分泌前列环素(PGI2)和内皮素-1(ET-1)的影响,以及α-硫辛酸(α-LPA)对其的影响。方法采用酶消化法收集人脐静脉内皮细胞,经原代培养后随机分为4组:正常对照组、AGES组、正常+AGES组和AGES+α-LPA组;放免法测定培养24、48、72 h时培养基中的PGI2和ET-1含量。结果①AGES组较正常对照组PGI2显著下降(P<0.05),ET-1显著升高(P<0.05);②AGES+α-LPA组较AGES组PGI2显著升高(P<0.05),ET-1显著下降(P<0.01)。结论 AGES对血管内皮细胞有损伤作用,α-LPA可减轻这种损伤,提示抗氧化剂可用于保护血管内皮细胞。
Objective To investigate the effects of AGEs on the secretion of prostacyclin (PGI2) and endothelin-1 (ET-1) in human endothelial cells and the effect of α-lipoic acid (α-LPA) on it. Methods The human umbilical vein endothelial cells were collected by enzymatic digestion and divided into 4 groups: normal control group, AGES group, normal + AGES group and AGES + α-LPA group after primary culture. The content of PGI2 and ET-1 in the medium at 72 h. Results ① Compared with normal control group, the levels of PGI2 in the three groups were significantly decreased (P <0.05) and the levels of ET-1 in the two groups were significantly increased (P <0.05) Significantly decreased (P <0.01). Conclusion AGES can damage vascular endothelial cells, α-LPA can reduce this damage, suggesting that antioxidants can be used to protect vascular endothelial cells.