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目的:研究细胞色素C(Cyt-C)和Survivin在结肠腺癌中的表达及其与临床病理特征的关系,并探讨Cyt-C和Survivin在结肠腺癌中的表达的相关性。方法:采用手工组织芯片技术及免疫组织化学技术(S-P法)检测59例结肠腺癌和10例正常结肠组织中Cyt-C和Survivin的表达水平。结果:Survivin和Cyt-C在正常结肠中的表达率分别为20.0%和30.0%,在结肠腺癌中的表达率分别为69.5%和78.0%,Survivin蛋白和Cyt-C在结肠腺癌中的表达增加(P<0.05);Cyt-C在结肠癌中的表达和肿瘤细胞的分化程度成正相关(P<0.05);Survivin蛋白在结肠癌中的表达和肿瘤细胞的分化程度成负相关(P<0.05);两者与浸润深度,肿瘤的大小,患者的年龄,淋巴结有无转移均无相关性(P>0.05)。结论:Survivin和Cyt-C在结肠癌中表达增加提示Survivin和Cyt-C对结肠癌的发生发展起着重要作用。
Objective: To study the expression of cytochrome C (Cyt-C) and Survivin in colorectal adenocarcinoma and its relationship with clinicopathological features, and to investigate the correlation between the expression of Cyt-C and Survivin in colonic adenocarcinoma. METHODS: The expression of Cyt-C and Survivin in 59 cases of colon adenocarcinoma and 10 cases of normal colon tissues were detected by manual tissue microarray technique and immunohistochemical technique (S-P method). Results: The expression rates of Survivin and Cyt-C in normal colon were 20.0% and 30.0%, respectively. The expression rates in colonic adenocarcinoma were 69.5% and 78.0%, respectively. Survivin protein and Cyt-C were detected in colon adenocarcinoma. The expression was increased (P<0.05); the expression of Cyt-C in colon cancer was positively correlated with the degree of tumor cell differentiation (P<0.05); the expression of Survivin protein in colon cancer was negatively correlated with the degree of differentiation of tumor cells (P <0.05); Both had no correlation with depth of invasion, tumor size, patient age, and lymph node metastasis (P>0.05). Conclusion: The increased expression of survivin and Cyt-C in colon cancer suggests that Survivin and Cyt-C play an important role in the development of colon cancer.