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目的检测趋化因子CCL5及其受体在浸润性乳腺癌组织中的表达并探讨其临床意义。方法应用SYBR GreenⅠreal time-PCR定量检测33例浸润性乳腺癌患者癌组织及其癌旁组织的CCL5及其受体CCR1、CCR3、CCR5 mRNA的表达。结果各期癌组织CCR1及CCR3 mRNA的相对表达量与癌旁正常组织差异无统计学意义(P>0.05),CCR5及CCL5 mRNA的相对表达量均高于癌旁正常组织(P<0.05),Ⅰ期和Ⅱ期乳腺癌组织CCL5 mRNA的表达量差异无统计学意义(P>0.05),Ⅲ期乳腺癌组织CCL5 mRNA的相对表达量为2.477,Ⅳ期乳腺癌组织CCL5 mRNA的相对表达量最高(3.710)。CCR5及CCL5 mRNA的表达高度相关(Pearson相关系数为0.971)。结论趋化因子CCL5及其受体CCR5 mRNA在Ⅳ期乳腺癌组织中的表达水平最高,CCL5可能以自分泌方式与受体CCR5结合促进乳腺癌生长和转移。
Objective To detect the expression of chemokine CCL5 and its receptor in invasive breast cancer and to investigate its clinical significance. Methods The expression of CCR5, CCR3 and CCR5 mRNA in 33 cases of invasive breast cancer and their adjacent tissues were detected by SYBR GreenⅠreal time-PCR. Results The relative expression of CCR1 and CCR3 mRNA in each stage had no statistical significance (P> 0.05), and the relative expression levels of CCR5 and CCL5 mRNA were significantly higher than those in adjacent normal tissues (P <0.05) There was no significant difference in the expression of CCL5 mRNA in stage Ⅰ and stage Ⅱ breast cancer (P> 0.05). The relative expression level of CCL5 mRNA in stage Ⅲ breast cancer was 2.477, and the relative expression level of CCL5 mRNA in stage Ⅳ breast cancer was the highest (3.710). CCR5 and CCL5 mRNA expression was highly correlated (Pearson correlation coefficient of 0.971). Conclusions CCL5 and its receptor CCR5 mRNA have the highest expression in stage Ⅳ breast cancer. CCL5 may promote the growth and metastasis of breast cancer by binding with receptor CCR5 in autocrine mode.