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目的:探讨HMGB1对巨噬细胞免疫功能的影响。方法:梯度浓度HMGB1处理小鼠腹腔巨噬细胞,或将小鼠随机分为生理盐水对照、24h和48h高与低剂量HMGB1注射组,腹腔注射0.2μg或20μg HMGB1,或生理盐水。检测巨噬细胞吞噬功能和I-A/E表达。结果:10μg/L HMGB1刺激6~12h,巨噬细胞吞噬功能较其他剂量组明显增强(P<0.05或P<0.01)。HMGB1对培养巨噬细胞I-A/-E表达无影响。高剂量HMGB1攻击小鼠24h,其腹腔巨噬细胞吞噬能力明显降低(P<0.05);低剂量HMGB1攻击48h,巨噬细胞I-A/-E表达明显上调(P<0.05或P<0.01)。结论:高剂量HMGB1抑制巨噬细胞吞噬功能,低剂量HMGB1增强巨噬细胞免疫功能。
Objective: To investigate the effect of HMGB1 on immune function of macrophages. METHODS: Mouse peritoneal macrophages were treated with gradient concentration of HMGB1, and mice were randomly divided into normal saline control group, high and low dose HMGB1 injection groups at 24h and 48h, intraperitoneal injection of 0.2μg or 20μg HMGB1, or normal saline. Macrophage phagocytosis and I-A / E expression were examined. Results: The phagocytosis of macrophages was significantly enhanced (P <0.05 or P <0.01) after stimulation with 10 μg / L HMGB1 for 6-12 h. HMGB1 had no effect on I-A / -E expression in cultured macrophages. The macrophage phagocytosis of macrophages was significantly decreased after high-dose HMGB1 challenge (P <0.05), and the expression of I-A / -E was up-regulated macrophages (P <0.05 or P <0.01) 48 h after low dose HMGB1 challenge. Conclusion: High dose HMGB1 can inhibit macrophage phagocytosis and low dose HMGB1 enhance macrophage immune function.