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本文介绍了测定α-脂蛋白胆固醇含量的方法,以及此种方法在检查脂质及脂蛋白代谢障碍中的诊断意义。测定方法将1毫升血清或血浆放入置于冰浴中的离心管中,加入40微升肝素溶液(5,000单位/毫升),混匀,再加入50微升1M MnCl_2溶液,振摇混匀,冰浴中放置30分钟。在4-6℃离心30分钟(2,500转/分),吸取上清液测定其中胆固醇含量。为了对高脂蛋白血症的分布作流行病学调查,作者在没有局部缺血性心脏病表现的40～50岁的200名男性中,作代表性抽样,以测定此亚人群胆固醇的正常值。对151例局部缺血性心脏病患者的α-脂蛋白胆固醇、血脂和β-脂蛋白的浓度作了统计
This article describes the determination of alpha-lipoprotein cholesterol content, as well as the diagnostic value of this method in the examination of lipid and lipoprotein metabolism disorders. Assay Method Place 1 ml of serum or plasma into a centrifuge tube placed in an ice bath, add 40 μl of heparin solution (5,000 units / ml), mix well, add 50 μl of 1M MnCl 2 solution, shake and mix well, Leave in ice bath for 30 minutes. Centrifuged at 4-6 ° C for 30 minutes (2,500 rpm), and the supernatant was aspirated to determine the cholesterol content. To make an epidemiological investigation of the distribution of hyperlipoproteinemia, the authors used a representative sample of 200 men aged 40 to 50 years without ischemic heart disease to determine the normal level of cholesterol in this sub-population . 151 patients with ischemic heart disease in patients with alpha-lipoprotein cholesterol, lipids and beta-lipoprotein concentration statistics