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目的:观察苍术白虎汤对大鼠急性痛风性关节炎(acute gouty arthritis,GA)的疗效并探讨其机制。方法:36只大鼠分为苍术白虎汤高、中、低剂量组,西药组,模型组和对照组。建立大鼠急性痛风性关节炎模型,对照组注射等量生理盐水。以缚线法测量右侧踝关节同一部位周径(取均值),评估其关节肿胀度;于造模72 h后腹腔静脉采血,ELISA法测定其血清IL-1b、TNF-α水平,对血浆白细胞进行计数。结果:苍术白虎汤可显著降低急性痛风性关节炎大鼠关节肿胀度,降低其血清IL-1b、TNF-α水平及血浆白细胞,以高剂量组最为明显(P<0.01)。结论:苍术白虎汤可通过降低IL-1b与TNF-α表达、抑制白细胞增生而发挥较好的抗急性痛风性关节炎作用。
Objective: To observe the effect of Cangzhu Baihu decoction on acute gouty arthritis (GA) in rats and its mechanism. Methods: Thirty-six rats were divided into high, medium and low dose of herb Baihu Tang, western medicine group, model group and control group. The rat model of acute gouty arthritis was established, and the control group was injected with the same amount of normal saline. The circumference of the same part of the right ankle joint (mean value) was measured by the tethering method and the degree of swelling of the joint was evaluated. Blood was collected from the abdominal cavity 72 h after model establishment, and the levels of IL-1b and TNF-α were measured by ELISA. WBC count. Results: Cangzhu Baihu decoction can significantly reduce the degree of joint swelling, reduce the levels of serum IL-1b, TNF-α and plasma leukocyte in the rats with acute gouty arthritis, especially in the high dose group (P <0.01). Conclusion: Cangsai Baihu Decoction can play a better anti-acute gouty arthritis effect by reducing the expression of IL-1b and TNF-α and inhibiting leukocyte proliferation.