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[目的]研究低硒大鼠对不同剂量的亚硒酸钠和富硒酵母的吸收利用效果。[方法]用低硒饲料(硒含量≤0.03μg/g)喂养大鼠建立低硒动物模型,然后分4个剂量2、4、8、16μg/kg体重灌胃,连续灌1个月,测定各组大鼠的血、肝和肾的硒含量,以及肝、肾的谷胱甘肽过氧化物酶(GSH-Px)活性及硫氧还蛋白还原酶(TrxR)含量。[结果]与对照组相比,在补硒4～8μg/kg体重的范围内,亚硒酸钠和富硒酵母均可显著提高血、肝和肾的硒含量(P﹤0.001,P﹤0.01,P﹤0.05),并且富硒酵母提高肝、肾硒含量的效果优于亚硒酸钠(P﹤0.05);富硒酵母组的肝、肾GSH-Px活性及TrxR含量与对照组相比均显著增加(P﹤0.001,P﹤0.01,P﹤0.05),其提高GSH-Px活性、增加TrxR含量的程度均高于亚硒酸钠(P﹤0.001,P﹤0.01)。[结论]在补硒4～8μg/kg体重的范围内,大鼠对有机硒富硒酵母的吸收利用较好。
[Objective] To study the absorption and utilization of sodium selenite and selenium-enriched yeast in low-selenium rats. [Method] The low selenium animal model was fed with low selenium diet (selenium content≤0.03μg / g), then the rats were fed with 4, 8, 8 and 16μg / kg body weight by gavage for 1 month, Selenium content in blood, liver and kidney of rats in each group, glutathione peroxidase (GSH-Px) activity and the content of thioredoxin reductase (TrxR) in liver and kidney were measured. [Result] Compared with the control group, both selenium selenite and selenium-enriched yeast could significantly increase selenium content in blood, liver and kidney (P <0.001, P <0.01) in the range of 4 ~ 8μg / , P <0.05), and selenium-enriched yeast was superior to sodium selenite in increasing liver and kidney selenium content (P <0.05). Compared with the control group, the activities of GSH-Px and TrxR in selenium-enriched yeast group (P <0.001, P <0.01, P <0.05). The activity of GSH-Px and the content of TrxR were all higher than that of sodium selenite (P <0.001, P <0.01). [Conclusion] The absorption and utilization of selenium-enriched organic selenium in rats were better in the range of 4 ~ 8μg / kg body weight of selenium.