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AIM:To study the effect of Ginkgo biloba extract (EGb 761)containing 22-27% flavonoids (ginkgo-flavone glycosides)and 5-7% terpenoids (ginkgolides and bilobalides) on cellproliferation and cytotoxicity in human hepatocellularcarcinoma (HCC) cells.METHODS:Human HCC cell lines (HepG2 and Hep3B) wereincubated with various concentrations (0-1000 mg/L) ofEGb 761 solution.After 24 h incubation,cell proliferation andcytotoxicity were determined by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay and lactate dehydrogenase (LDH)release,respectively.After 48 h incubation,the expressionof proliferating cell nuclear antigen (PCNA) and p53 proteinwas measured by Western blotting.RESULTS:The results showed that EGb 761 (50-1000 mg/L)significantly suppressed cell proliferation and increased LDHrelease (P<0.05) in HepG2 and Hep3B cells compared withthe control group.The cell proliferation of HepG2 and Hep3Bcells treated with EGb 761 (1000 mg/L) was 45% and 39%of the control group (P<0.05),respectively.LDH release ofHepG2 cells without and with EGb 761 (1000 mg/L) treatmentwas 6.7% and 37.7%,respectively,and that of Hep3B cellswithout and with EGb 761 (1000 mg/L) treatment was 7.2%and 40.3%,respectively.The expression of PCNA and p53protein in HepG2 cells treated with EGb 761 (1000 mg/L)was 85% and 174% of the control group,respectively.CONCLUSION:Ginkgo biloba extract significantly can suppressproliferation and increase cytotoxicity in HepG2 and Hep3Bcells.Additionally,Ginkgo biloba extract can decrease PCNAand increase p53 expression in HepG2 cells.
AIM: To study the effect of Ginkgo biloba extract (EGb 761) containing 22-27% flavonoids (ginkgo-flavone glycosides) and 5-7% terpenoids (ginkgolides and bilobalides) on cellproliferation and cytotoxicity in human hepatocellular carcinoma (HCC) cells. METHODS : Human HCC cell lines (HepG2 and Hep3B) wereincubated with various concentrations (0-1000 mg / L) of EGb 761 solution. After 24 h incubation, cell proliferation and cytotoxicity were determined by 3- (4,5-dimethylthiazol- After 48 h incubation, the expression of proliferating cell nuclear antigen (PCNA) and p53 (5-carboxymethoxyphenyl) -2- (4-sulfophenyl) -2H- tetrazolium (MTS) assay and lactate dehydrogenase (LDH) protein was measured by Western blotting. RESULTS: The results showed that EGb 761 (50-1000 mg / L) significantly suppressed cell proliferation and increased LDH release (P <0.05) in HepG2 and Hep3B cells compared with the control group. The cell proliferation of HepG2 and Hep3Bcells treated with EGb 761 (1000 mg / L) was 4 5% and 39% of the control group (P <0.05), respectively. LDH release ofHepG2 cells without and with EGb 761 (1000 mg / L) treatmentwas 6.7% and 37.7% respectively, and that of Hep3B cells with out and with EGb 761 (1000 mg / L) was was 7.2% and 40.3%, respectively. The expression of PCNA and p53 protein in HepG2 cells treated with EGb 761 (1000 mg / L) was 85% and 174% of the control group, Ginkgo biloba extract significantly can suppressproliferation and increase cytotoxicity in HepG2 and Hep3Bcells. Additionally, Ginkgo biloba extract can decrease PCNA and increase p53 expression in HepG2 cells.