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目的:探讨罗格列酮对人子宫肌瘤细胞增殖和凋亡的影响及可能机制。方法:不同浓度的罗格列酮(10-8,10-7和10-6mol/L)处理原代培养的人子宫肌瘤细胞24,48和72h。用四甲基偶氮唑盐(methyl thiazolyl tet-razolium,MTT)法测定细胞生长曲线,用流式细胞术测定细胞凋亡率,采用放射免疫法测定培养液中雌二醇水平,实时定量PCR和Western免疫印迹分别检测雌激素受体α和β的表达。结果:罗格列酮(10-8,10-7和10-6mol/L)处理48和72h后以浓度和时间依赖的方式抑制了子宫肌瘤细胞的增殖,而罗格列酮(10-8,10-7和10-6mol/L)处理24h对子宫肌瘤细胞增殖的影响没有显著性。罗格列酮(10-8,10-7和10-6mol/L)处理72h以浓度依赖的方式促进了子宫肌瘤细胞的凋亡,同时以浓度依赖的方式降低了子宫肌瘤细胞雌二醇的分泌和雌激素受体α和βmRNA和蛋白的表达。结论:罗格列酮可抑制子宫肌瘤细胞增殖,促进其凋亡,其机制与罗格列酮降低子宫肌瘤细胞雌二醇的分泌和减少子宫肌瘤细胞中雌激素受体α和β的表达有关。
Objective: To investigate the effects of rosiglitazone on the proliferation and apoptosis of human uterine fibroids and its possible mechanism. Methods: Primary cultured human uterine fibroids were treated with different concentrations of rosiglitazone (10-8, 10-7 and 10-6 mol / L) for 24, 48 and 72 h. The cell growth curve was determined by methyl thiazolyl tet-razolium (MTT) method. The apoptosis rate was determined by flow cytometry. The estradiol level in the culture medium was determined by radioimmunoassay. The real-time quantitative PCR Western blot was used to detect the expression of estrogen receptor alpha and beta respectively. RESULTS: Rosiglitazone (10-8, 10-7 and 10-6 mol / L) inhibited uterine myoma cell proliferation in a concentration- and time-dependent manner at 48 and 72 h, whereas rosiglitazone (10-, 8,10-7 and 10-6mol / L) for 24 h on the proliferation of uterine fibroids was not significant. Rosiglitazone (10-8, 10-7 and 10-6mol / L) for 72h promoted uterine myoma cell apoptosis in a concentration-dependent manner and reduced uterine fibroids estrogen Alcohol secretion and estrogen receptor alpha and beta mRNA and protein expression. CONCLUSION: Rosiglitazone can inhibit the proliferation of uterine fibroids and promote their apoptosis. The mechanism of rosiglitazone is that Rosiglitazone can reduce the secretion of estradiol in uterine fibroids and decrease the expression of estrogen receptor alpha and beta Related to the expression.