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采用桥联酶标APAAP染色技术检测无先兆偏头痛（MWA）患者淋巴细胞亚群CD3+、CD4+、CD8+、CD15+、CD19+的变化，用敏感细胞株杀伤试验及依赖细胞株增殖试验（MTT比色法）分别检测血浆TNF及IL－6水平的变化。结果显示：偏头痛患者外周血白细胞及淋巴细胞数均在正常范围内，发作期白细胞数较缓解期明显升高（P＜0．05）；而淋巴细胞数各组间差异无显著意义。CD3+、CD4+、CD8+、CD4+／CD8+比率MWA组与对照组间差异均无显著性意义，但CD15+以细胞数则显著低于对照组（P＜0．01）。MWA组血浆TNF、IL－6水平显著高于对照组（P＜0．01），且发作期高于缓解期（P＜0．05）。对4例患者进行连续检测结果显示，发作期、缓解期血浆TNF与IL-6水平之间呈正相关（r＝0．9232，P＜0．01）。以上结果提示MWA患者存在细胞免疫缺陷，炎性细胞因子IL－6、TNF在其发病中可能起一定作用。
The level of CD3 +, CD4 +, CD8 +, CD15 + and CD19 + of lymphocyte subsets in patients with ametropic migraines (MWA) was detected by using the enzyme linked APAAP assay. The cell cycle was detected by MTT colorimetric assay ) Were detected in plasma TNF and IL-6 levels. The results showed that the number of peripheral leukocytes and lymphocytes in patients with migraine was within the normal range, and the number of leukocytes in the seizure stage was significantly higher than that in the remission stage (P <0.05). There was no significant difference in the number of lymphocytes between the groups. CD3 +, CD4 +, CD8 +, CD4 + / CD8 + ratios between MWA group and control group had no significant difference, but the number of CD15 + cells was significantly lower than the control group (P <0.01). The levels of plasma TNF and IL-6 in MWA group were significantly higher than those in control group (P <0.01), and were significantly higher than those in remission stage (P <0.05). Continuous examination of 4 patients showed that there was a positive correlation between plasma TNF and IL-6 levels during the onset and remission (r = 0.9232, P <0.01). The above results suggest that cellular immune deficiency exists in MWA patients, and inflammatory cytokines IL-6 and TNF may play a role in their pathogenesis.