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目的探讨吸入麻醉剂对急性肾缺血-再灌注损伤的保护作用及其机制。方法SD大鼠36只,随机分为对照(S)组、缺血-再灌注(IR)组、异氟醚(SIO)组和七氟醚(Sevo)组,每组12只。建立大鼠急性肾缺血-再灌注损伤模型,缺血-再灌注后3h分别检测血清尿素氮(BUN)、肌酐(Cr)、超氧化物歧化酶(SOD)、丙二醛(MDA)及观察肾组织的病理学变化。结果与S组比较,IR组血清BUN、Cr水平显著增加(P<0.05),ISO组和Sevo组BUN、Cr低于IR组(P<0.05);与IR组比较,ISO组和Sevo组SOD显著升高,MDA显著降低(P<0.05);IR组和ISO组和Sevo组血清IL-6、TNF-α显著大于S组(P<0.05),但与IR组比较,ISO组和Sevo组均低于低于IR组(P<0.05);ISO组和Sevo组肾组织病理损伤分级明显低于IR组;ISO组和Sevo组比较各项指标均无显著性差异(P>0.05)。结论吸入麻醉药对大鼠急性肾缺血-再灌注损伤具有保护作用,抑制氧自由基反应和降低IL-6、TNF-α等细胞因子水平可能是其重要机制。
Objective To investigate the protective effect of inhalation anesthetics on acute renal ischemia-reperfusion injury and its mechanism. Methods Thirty - six SD rats were randomly divided into control (S) group, ischemia - reperfusion (IR) group, isoflurane group (SIO) and sevoflurane group (n = 12). Acute renal ischemia / reperfusion injury model was established in rats. Serum urea nitrogen (BUN), creatinine (Cr), superoxide dismutase (SOD), malondialdehyde (MDA) Observe the pathological changes of kidney tissue. Results Compared with S group, BUN and Cr in serum of IR group increased significantly (P <0.05), BUN and Cr in ISO group and Sevo group were lower than IR group (P <0.05) (P <0.05). The serum levels of IL-6 and TNF-α in IR group, ISO group and Sevo group were significantly higher than those in S group (P <0.05) (P <0.05). The grading of renal tissue in ISO group and Sevo group was significantly lower than that in IR group. There was no significant difference between ISO group and Sevo group (P> 0.05). Conclusion Inhaled anesthetics have a protective effect on acute renal ischemia-reperfusion injury in rats. Inhibition of oxygen free radical reaction and reduction of cytokines such as IL-6 and TNF-α may be important mechanisms.