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目的:对细胞系中支原体的感染情况进行检测并分型。方法:利用支原体 16S~23S保守区域扩增来检出细胞系中支原体感染率,并利用 16S~23S保守区间的间隔区(spacer)长度不同进行巢式PCR扩增,结合测序达到分型的目的。结果:在检测的 22株细胞系中,有 77. 3% (17株)的细胞系有支原体感染,其中 5株有 2种或 2种以上支原体感染,在分布上以M.fermentans和M.hyorhinis支原体多见。结论:支原体在细胞系中的高感染率提示,在以细胞系作为模型的研究中,对支原体感染所造成的影响应有充分认识。检测及分型技术的建立为进一步研究不同型别支原体的致病力提供了可能。
Objective: To detect and classify the mycoplasma infection in cell lines. Methods: Mycoplasma infection rate was detected by 16S ~ 23S conservative region amplification in mycoplasma. The nested PCR amplification was carried out by using spacer length of 16S ~ 23S conservative interval, and then the sequencing was performed to achieve the purpose of typing . Results: Of the 22 cell lines tested, 77.3% (17) of the cell lines were infected with mycoplasma, of which 5 had 2 or more mycoplasma infection, the distribution of M.fermentans and M. Mycoplasma hyorhinis more common. Conclusions: The high prevalence of mycoplasma in cell lines suggests that the effects of mycoplasma infection should be well understood in studies using cell lines as a model. The establishment of detection and typing technology has provided the possibility for further study on the pathogenicity of different types of Mycoplasma.