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目的 通过研究山莨菪碱 (ADM ,6 5 4 2 )预防大鼠肺水肿时 ,对动脉血浆和支气管肺泡洗涤液 (BAL)中的PGE2和LTC4 含量的影响 ,探讨山莨菪碱防治肺水肿的部分机制。方法 先从股静脉注射山莨菪碱 (30mg·kg-1)作为预防 ,再注射肾上腺素 (0 .3mg·kg-1) ,30min后收集动脉血浆和BAL ,用放射免疫法分别测定正常对照组、肺水肿组、山莨菪碱预防组的PGE2 和LTC4 的含量。结果 肺水肿组动脉血浆中的PGE2 和LTC4 的含量、LTC4 与PGE2 比值高于正常对照组 (P <0 0 1) ,BAL中的PGE2 含量低于正常对照组 (P <0 0 5 ) ,LTC4 的含量、LTC4 与PGE2 比值高于正常对照组 (P <0 0 1) ;山莨菪碱预防组动脉血浆和BAL中PGE2和LTC4 含量、LTC4 与PGE2 比值均低于肺水肿组 (P <0 0 1)。结论 山莨菪碱能抑制肺水肿时PGE2 和LTC4 的释放 ,从而改善血管的通透性 ,是缓解肺水肿的机制之一
Objective To study the effect of anisodamine (ADM, 6 5 4 2) on the PGE2 and LTC4 levels in pulmonary arteries and bronchoalveolar lavage fluid (BAL) in the prevention of pulmonary edema in rats and to explore the effect of anisodamine in the prevention and treatment of pulmonary edema mechanism. Methods Anisodamine (0.3 mg · kg-1) was injected intravenously from femoral vein at 30 mg · kg-1. After 30 min, arterial plasma and BAL were collected, and radioimmunoassay , Pulmonary edema group and anisodamine preventive group. Results The contents of PGE2 and LTC4, the ratio of LTC4 and PGE2 in pulmonary arteries were higher in the pulmonary edema group than those in the normal control group (P <0.01). The content of PGE2 in the BAL was lower than that in the normal control group (P <0.05) (P <0.01). The content of PGE2 and LTC4 in arterial plasma and BAL in the group of Anisodamine prevention and the ratio of LTC4 to PGE2 were lower than those in the group of pulmonary edema (P <0 0) 1). Conclusion Anisodamine can inhibit the release of PGE2 and LTC4 during pulmonary edema and thus improve the permeability of blood vessels and is one of the mechanisms to alleviate pulmonary edema