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利用自主开发研制的作物脂肪氧化酶同功酶快速检测技术 ,筛选出Lox -1、Lox -2缺失材料皖鉴 2 0 90、皖鉴 10 2 4、皖鉴3 0 3 3等。为了进一步研究鉴定脂质氧化酶缺失对稻谷陈化、仓储害虫的影响 ,将 3个Lox缺失材料 (DawDam、冲腿、皖鉴 2 0 90 )和其他 3个Lox不缺失品种 (早籼 14、中籼 898、晚粳M3 12 2 ) ,在一定的储藏条件、温度、时间下 ,调查了仓储害虫危害情况和种子生活率。结果表明 :①在储藏过程中 ,品种间仓储害虫危害差异较大。在 5个籼稻品种中 ,Lox -3缺失种质DawDam和冲腿的虫蛀率约 5 % ,为最低 ;中籼 898、皖鉴 2 0 90、早籼 14的虫蛀率在 13 .8%～ 19.5 % ;粳稻品种晚粳M3 12 2的虫蛀率最高 ,达 3 3 .5 %。因此 ,Lox -3缺失种质的虫蛀率的发生较其他品种低 2 .5～ 6.5倍。②随着储藏期的增加 ,种子发芽率呈下降趋势。但是Lox -1、Lox -2缺失的皖鉴 2 0 90 ,至调查时储存 42个月 ,发芽率几乎没有变化 ,仍保持在 98.5 % ,成苗率高达 85 %。由此表明 ,Lox -1、Lox -2的缺失对保持种子生活力和延缓稻谷陈化变质具有重要的作用 ,可能是影响种子长寿命的关键基因
Using self-developed rapid detection of lipoxygenase isozymes, Lox-1 and Lox-2 missing materials were screened from Anhui Province, Anhui Province, Anhui Province, Anhui Province, Liaoning Province, and Anhui Province. In order to further study the effects of the deletion of lipid oxidase on rice aging and storage pests, three Lox missing materials (DawDam, Hongqi, Wanjian2090) and three other Lox non-deletion cultivars (Zaozhan14, Medium indica rice 898, late japonica rice M312 2) under certain storage conditions, temperature and time, investigated the harm of storage pests and the seed life rate. The results showed that: ①During the storage, there were significant differences between the varieties of storage pests. In 5 indica rice varieties, the rate of moth-eaten of DawDam and Chong-Le of Lox-3-deficient germplasm was about 5%, which was the lowest. The medium-indica 898, Wanjian 2090, ~ 19.5% respectively. The japonica rice M3 12 2 possessed the highest moth-eaten rate of 33.3%. Therefore, the loss rate of Lox -3 deletion germplasm was 2.5 ~ 6.5 times lower than that of other varieties. ② With the increase of storage period, the seed germination rate showed a downward trend. However, Lox-1 and Lox-2-deficient Wanjian2090 were stored at the survey for 42 months with almost no change in germination rate, remained at 98.5% and their seedling rate was as high as 85%. The results indicated that the deletion of Lox -1 and Lox -2 played an important role in maintaining seed viability and alleviating the aging of rice, which may be the key genes that affect the long-life of seed