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掘氏疫霉(Phytophthora drechsleri)种内菌株直接交配产生的卵孢子分别用0.1%KMnO_4处理20分钟和0.3%H_2O_2处理2分钟在S+L培养基上(26℃)培养7天萌发率>70%。H_2O_2是本研究首次报道的一种刺激掘氏疫霉卵孢子萌发的处理剂,其效果略优于KMnO_4。用KMnO_4和H_2O_2处理均可有效地抑制卵孢子悬浮液中菌丝片段及菌丝膨大体的萌发生长。在一定时期内卵孢子萌发率随卵孢子保存时间的增加而增加,保存30天和45天的卵孢子分别用KMnO_4和H_2O_2处理萌发率最高。卵孢子保存期间有无光照对萌发率无显著影响,但卵孢子荫发时给予光照对萌发有明显的促进作用,保存30天的卵孢子在光照下萌发率为60—70%,在黑暗中仅0—16%。卵孢子萌发过程中的光照条件以黑光灯8小时,日光灯16小时交替连续照射7天效果最好,其次为黑光灯单独连续光照,以日光灯单独照射效果较差。所测定的6种培养基中以S+L培养基对卵孢子萌发的效果最好,其次为V_(?)+L和WA+L。蜗牛酶、纤维素酶、土壤浸出液和黄瓜果提取液对掘氏疫霉卵孢子萌发无明显刺激作用。
The oospores produced by direct inoculation with Phytophthora drechsleri strains were treated with 0.1% KMnO 4 for 20 minutes and 0.3% H 2 O 2 for 2 minutes, respectively, on S + L medium (26 ℃) for 7 days. The germination rate was> 70 %. H 2 O 2 is the first treatment reported in this study to stimulate spore germination of Phytophthora sojae, and its effect is slightly better than that of KMnO 4. Both KMnO_4 and H_2O_2 treatment could effectively inhibit the germination and growth of mycelial fragments and hyphae in oospores. The germination rate of oospores increased with the preservation time of oospores in a certain period, and the germination rate of oospores stored for 30 days and 45 days were the highest with KMnO 4 and H 2 O 2, respectively. There was no significant effect of light on the germination rate during the storage of oospores, but the illumination of oospores caused by germination had obvious promotion effect on germination. The germination rate of oospores stored for 30 days was 60-70% in the dark, Only 0-16%. During the germination of oospores, the illumination conditions were as follows: black light for 8 hours, fluorescent light for 16 hours and continuous light for 7 days, followed by black light for continuous and single light irradiation. Among 6 tested media, S + L medium had the best effect on spore germination, followed by V _ (?) + L and WA + L. Snail enzyme, cellulase, soil leachate and cucumber extract had no obvious stimulating effects on the spore germination of Phytophthora dunnii.