In a surfactant assisted biodegradation process, the choice of surfactant(s) is of crucial importance. The question is: does the type of surfactant (i.e. chemical family) affect the biodegradation process at fixed hidrophillic-lypofillic balance HLB values? Microcosm assessments were developed using contaminated soil, with around of 5000 mg/kg of hydrocarbons as TPH-diesel. Mixtures of three nonionic surfactants were employed to get a wide range of specific HLB values. Tween20 and Span20 were mixed in the appropriate proportions to get HLB values between 8.6 and 16.7. Tween/Span60 mixtures reached HLB values between 4.7 and 14.9. Finally, Tween/Span80 combinations yielded HLB values between 4.3 and 15. TPH-diesel biodegradation was measured at the beginning, and after 8 weeks, as well as the FCU/grsoil, as a measure of microorganisms′ development during the biodegradation period. A second aim of this work was to assess the use of guar gum as a biodegradation enhancer instead of synthetic products. The conclusions of this work are that surfactant chemical family, and not only the HLB value clearly affects the assisted biodegradation rate. Surfactants synergism was clearly observed. Regarding the use of guar gum, no biodegradation enhancement was observed for the three assessed concentrations i.e., 2, 20, and 200 mg/kg, respectively. On the contrary, TPH-diesel removal was lower as the gum concentration increased. It is quite possible that guar gum was used as a microbial substrate.