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目的:分析实验性自身免疫性甲状腺疾病大鼠血清核转录因子(NF-κB)及其相关因子表达情况。方法:采用实验性自身免疫性甲状腺炎(experimental autoimmune thyroiditis,EAT)大鼠模型,分为3组:正常对照组,碘剂喂养组和碘剂联合抗原免疫组,比较3组的差异。结果:碘剂喂养组血清甲状腺球蛋白抗体(Tg Ab)、甲状腺微粒体抗体(Tm Ab)和甲状腺过氧化物酶抗体(TPOAb)水平自第2周逐渐升高,第8周达到高峰(P<0.05)。碘剂联合抗原免疫组血清Tg Ab、Tm Ab和TPOAb水平自第2周开始升高,第8周达到高峰(P<0.05)。碘剂喂养组血清甲状腺球蛋白抗体(Tg Ab)、血清甲状腺微粒体抗体(Tm Ab)和血清甲状腺过氧化物酶抗体(TPOAb)虽然低于碘剂联合抗原免疫组,但高于正常对照组(P<0.05);碘剂喂养组血清核转录因子(NF-κB)和血清肿瘤坏死因子(TNF-α)水平自第4周逐渐升高,第6周达到高峰(P<0.05)。碘剂联合抗原免疫组血清NF-κB和TNF-α水平自第2周开始升高,第6周达到高峰并高于碘剂喂养组(P<0.05)。碘剂喂养组血清(NF-κB)和血清肿瘤坏死因子(TNF-α)虽然低于碘剂联合抗原免疫组,但高于正常对照组(P<0.05)。结论:实验性自身免疫性甲状腺疾病大鼠血清核转录因子(NF-κB)及其相关因子有明显表达。
Objective: To analyze the expression of serum nuclear transcription factor (NF-κB) and related factors in experimental autoimmune thyroid disease rats. Methods: The experimental autoimmune thyroiditis (EAT) rat model was divided into three groups: the normal control group, the iodine-fed group and the iodine-combined antigen immunized group. The differences among the three groups were compared. Results: Serum levels of Tg Ab, Tm Ab and TPOAb in iodine-fed group increased gradually from the second week to peak (P <0.05). The levels of serum Tg Ab, Tm Ab and TPOAb in the immunized group were increased from the second week and reached the peak at the eighth week (P <0.05). Serum Tg Ab, Tm Ab and TPOAb in iodine-fed group were lower than those in immunized group, but higher than those in normal control group (P <0.05). The levels of serum NF-κB and TNF-α in iodine-fed group increased gradually from the 4th week to the 6th week (P <0.05). The serum levels of NF-κB and TNF-α in the immunized group were increased from the second week, reached the peak at the sixth week and were higher than those in the iodine-fed group (P <0.05). The level of serum NF-κB and serum tumor necrosis factor (TNF-α) in iodine-fed group were lower than those in immunized group, but higher than that in normal control group (P <0.05). Conclusion: Serum nuclear factor-kappa B (NF-κB) and its related factors are obviously expressed in experimental autoimmune thyroid disease rats.