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为探明‘峰后’葡萄在赤霉素(GA3)处理促使葡萄座果率增加原因和VvAG基因在葡萄坐果过程中的作用,在开花前10d用0.02mg/g的GA3和赤霉素合成抑制剂PAC处理葡萄花序并通过石蜡切片观察葡萄早期果实发育结构的变化,通过RT-PCR分析了VvAG基因的表达。结果显示:GA3处理提高了葡萄座果率,PAC处理则降低座果率,而PAC+GA3处理可以恢复葡萄正常坐果。GA3处理在开花后0~10d幼果纵横径大于对照,而PAC处理则小于对照。进一步分析发现,GA3处理后中果皮细胞明显大于对照,而PAC处理则小于对照,说明GA3处理导致开花后0~10d幼果增大的原因是由于细胞分裂和中果皮细胞增大的缘故。VvAG基因的表达呈现出组织特异性,VvAG2在叶片,茎和卷须中均有少量表达,VvAG1和VvAG3则表达量很低,在花和果实中VvAG1和VvAG2有较高的表达,表明这2个基因对于葡萄花发育和坐果是必需的。VvAG3在花和果实中的表达量相对较低,但在GA3处理后表达上调;相反,在PAC处理后,VvAG3表达量下调,表明VvAG3在坐果过程中的表达可能受到赤霉素的调控。
In order to find out the reason why post-peak grape increased the fruit rate of grape and the role of VvAG in grape fruit setting by GA3, GA3 and GA3 (0.02mg / g) were synthesized 10 days before flowering Inhibitor PAC treated grape inflorescence and observed the changes of grape early developmental structure through paraffin section. The expression of VvAG gene was analyzed by RT-PCR. The results showed that: GA3 treatment increased the fruit rate of grape, PAC reduced the fruit rate, while PAC + GA3 treatment could restore the normal fruit setting. Compared with the control, the vertical and horizontal diameter of young fruit in GA3 treatment was 0-10 days after anthesis, while the PAC treatment was less than the control. Further analysis showed that the mesocarp cells after GA3 treatment were significantly larger than the control, while the PAC treatment was less than the control, indicating that the reason GA3 treatment led to 0 to 10 days after flowering young fruit growth is due to cell division and mesocarp increased. VvAG gene expression showed tissue specificity, VvAG2 in leaves, stems and tendons in a small amount of expression, VvAG1 and VvAG3 expression was low, in flowers and fruits VvAG1 and VvAG2 have a higher expression, indicating that the two Genes are essential for grape flower development and fruit set. VvAG3 expression was relatively low in flowers and fruits, but was up-regulated after GA3 treatment. On the contrary, the expression of VvAG3 was down-regulated after PAC treatment, indicating that the expression of VvAG3 may be regulated by gibberellin during fruit setting.