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目的:观察豚鼠耳蜗丝束肌动蛋白的表达及分布情况以及条件声暴露后耳蜗毛细胞丝束肌动蛋白的表达有无改变,初步探讨耳蜗“坚韧化”现象的发生机制。方法:实验用豚鼠分组后,采用中心频率为1kHz、强度为95dBSPL的倍频程噪声对动物进行条件声暴露。条件声暴露结束后按实验设计的时间处死动物并进行耳蜗铺片及免疫荧光染色。应用激光扫描共聚焦显微镜观察柯替器荧光染色情况并对1kHz频率感音区的第3回柯替器外毛细胞(OHC)的荧光强度进行定量分析。结果:豚鼠耳蜗柯替器丝束肌动蛋白主要分布在内外毛细胞的静纤毛、表皮板,OHC顶部细胞侧壁,柱细胞头板。在底回柯替器OHC基底部,Deiters细胞指突,外柱细胞的胞体亦含有丰富的丝束肌动蛋白。不同时期的条件声暴露后,除条件声暴露1d组加休息5d组外,其他2组动物OHC的荧光强度均有明显变化。结论:①一定剂量的条件声暴露可导致耳蜗柯替器丝束肌动蛋白表达的改变。②除内外毛细胞的静纤毛、表皮板,柱细胞头板等既往报道的部位外,豚鼠耳蜗柯替器的外柱细胞胞体中也含有丰富的丝束肌动蛋白。③条件声暴露后耳蜗OHC丝束肌动蛋白浓度的减低可能与耳蜗“坚韧化”现象的产生有关。
OBJECTIVE: To investigate the expression and distribution of actin in cochlear filaments of guinea pigs and the changes of actin expression in cochlear hair cells after acoustic exposure, and to explore the mechanism of “toughening” of cochlea. METHODS: After the guinea pigs were grouped, the animals were exposed to conditioned sound using octave noise with a center frequency of 1 kHz and an intensity of 95 dB SPL. Animals were sacrificed at the end of the experimental period after exposure to conditioned acoustic exposure and cochlear implants and immunofluorescence staining. The confocal laser scanning confocal microscope was used to observe the fluorescence staining of the cuneata. The fluorescence intensity of the outer hair cells (OHC) of the third passage in the 1 kHz frequency sensitive area was quantitatively analyzed. Results: The ciliary actin of cochlear cochlear in guinea pigs mainly distributed in the ciliary wall, epidermis, the cell wall of the top of OHC and the head plate of the columnar cell. At the bottom of the OHC basal ganglia, Deiters cells indicate that the cells of the outer column cells are also rich in actin filaments. Exposed to different periods of time, the fluorescence intensity of OHC in the other two groups changed significantly except for the 1d group exposed to conditioned sound and the rest 5d group. CONCLUSIONS: ① A certain dose of conditioned acoustic exposure can cause changes of actin expression in the cochlear cochlear implant. ② In addition to the inner and outer hair cells of the static cilia, epidermal plate, cell head plate and other previously reported parts, guinea pig cochlear extracellular column cell body cells also contain rich filament actin. (3) The reduction of actin concentration in the OHC filament of cochlea after exposure may be related to the “toughening” of the cochlea.