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目的:系统研究太白山地区索骨丹化学成分,为进一步研究其药理作用提供理论依据。方法:采用硅胶柱色谱、凝胶柱色谱、重结晶等方法分离纯化化合物,并结合化合物的理化性质和波谱分析鉴定其结构。通过最小抑菌浓度法对所得化合物进行活性测试。结果:从索骨丹中分离得到8个化合物,分别为β-谷甾醇(1),鬼灯檠素(2),儿茶素(3),对羟基苯甲酸(4),3β-hydroxyolean-12-en-27-oic acid(5),没食子酸(6),琥珀酸(7),苹果酸甲酯(8)。结论:化合物4,5,7和8均为首次从该植物中分离得到,其中化合物5是首次从该属植物中分离得到,而且化合物5对大肠杆菌有较好的抑菌活性,最小抑菌浓度为15.6 mg·L-1。
OBJECTIVE: To systematically study the chemical constituents of Salvia bidentata in Taibai Mountain area, and to provide a theoretical basis for further study of its pharmacological effects. Methods: The compounds were isolated by silica gel column chromatography, gel column chromatography and recrystallization. Their structures were elucidated by physicochemical properties and spectral analysis. The resulting compounds were tested for activity by the minimum inhibitory concentration method. Results: Eight compounds were isolated from Cordyceps sinensis, which were β-sitosterol (1), ghrelin (2), catechin (3), p-hydroxybenzoic acid (4), 3β-hydroxyolean-12 -en-27-oic acid (5), gallic acid (6), succinic acid (7) and methyl malate (8). CONCLUSION: Compounds 4, 5, 7 and 8 were isolated from this plant for the first time. Among them, compound 5 was isolated from this genus for the first time, and compound 5 has good antibacterial activity against E. coli. The concentration of 15.6 mg · L-1.