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目的:探讨来源于施万细胞的人类听神经细胞瘤组织中是否存在髓鞘结构及其细胞学特点。方法:应用免疫荧光标记、Western blotting及电镜等方法检测听神经瘤组织中的髓鞘结构及重要髓鞘蛋白。结果:电镜结果发现:听神经瘤组织中虽然缺失成熟的轴索结构但可以检测到早期的髓鞘样结构。免疫荧光研究和Western blotting研究结果显示:听神经瘤组织中检测不到轴索的标志性抗体——神经丝200的表达。并且启动髓鞘化的前哨信号及髓鞘致密化的重要组成蛋白——髓鞘碱性蛋白在听神经瘤组织中也无法检测到。而一种非成熟施万细胞的标志抗体——神经生长因子受体p75,在听神经瘤组织中可以检测到其表达。结论:在听神经瘤组织中的施万细胞失去了完成髓鞘化进程和重新形成致密的髓鞘并包绕轴索的能力,处于前髓鞘化施万细胞阶段。
Objective: To investigate the existence of myelin structure and its cytological features in human Schwann cell-derived human acoustic neuroblastoma. Methods: Immunofluorescence staining, Western blotting and electron microscopy were used to detect myelin structure and important myelin protein in acoustic neuroma tissue. Results: Electron microscopy showed that although the mature axonal structure was missing in acoustic neuroma tissue, early myelin-like structure could be detected. Immunofluorescence studies and Western blotting results showed that the expression of neurofilament 200, an axonal marker antibody, was undetectable in acoustic neuroma tissue. And the myelin basic protein that initiates myelination of sentinel signal and densification of myelin is also undetectable in acoustic neuroma tissue. And a non-mature Schwann cell marker antibody - nerve growth factor receptor p75, can be detected in acoustic neuroma tissue expression. CONCLUSIONS: Schwann cells in acoustic neuroma tissue have lost the ability to complete myelination and re-form a dense myelin and wrap around the axon, at the stage of anterior myelinated Schwann cells.