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目的:探讨CDK4、p16在喉鳞状细胞癌中的表达。方法:采用免疫组织化学SP法检测30例喉鳞状细胞癌组织和20例癌旁组织中CDK4和p16的表达情况,并分析二者分别与喉鳞状细胞癌临床分期和病理分级的关系。结果:在喉鳞状细胞癌中CDK4、p16的表达率分别为63.3%、46.7%,在癌旁组织中CDK4、p16的表达率分别为25%、90%。CDK4在喉鳞状细胞癌中的表达高于癌旁组织(P<0.05),与病理分级、临床分期均无显著相关性(P>0.05);p16在喉鳞状细胞癌中的表达低于癌旁组织(P<0.05),与病理分级有相关性(P<0.05),与临床分期无显著相关性(P>0.05);CDK4与p16的表达呈负相关(rs=-0.786,P<0.05)。结论:p16的低表达和CDK4的高表达可能在喉鳞状细胞癌的发生发展中起重要作用,且p16的低表达可能作为判断肿瘤恶性程度的指标。
Objective: To investigate the expression of CDK4 and p16 in laryngeal squamous cell carcinoma. Methods: The expressions of CDK4 and p16 in 30 cases of laryngeal squamous cell carcinoma and 20 cases of paracancerous tissues were detected by immunohistochemical SP method. The relationship between the expression of CDK4 and p16 in clinical staging and pathological grade of laryngeal squamous cell carcinoma was analyzed. Results: The expression rates of CDK4 and p16 in laryngeal squamous cell carcinoma were 63.3% and 46.7%, respectively. The expression rates of CDK4 and p16 in adjacent tissues were 25% and 90% respectively. The expression of CDK4 in laryngeal squamous cell carcinoma was significantly higher than that in paracancerous tissues (P <0.05), but not in pathological grade and clinical stage (P> 0.05). The expression of p16 in laryngeal squamous cell carcinoma (P <0.05), and there was no significant correlation with clinical stage (P> 0.05). The expression of CDK4 was negatively correlated with the expression of p16 (rs = -0.786, P < 0.05). CONCLUSION: Low expression of p16 and high expression of CDK4 may play an important role in the occurrence and development of laryngeal squamous cell carcinoma, and the low expression of p16 may be used as an indicator of malignancy.