论文部分内容阅读
目的探讨不同压强持续性CO2气腹对结肠癌细胞体内侵袭转移能力的影响。方法建立体外气腹模型,将人结肠癌细胞株SW1116分为5组,分别在不同压强CO2气体[6、9、12、15mmHg(1mmHg=0.133kPa)]以及常规培养条件下暴露1h后,各自注入每组16只裸鼠腹腔(1×106个细胞/裸鼠)。2周后,各组裸鼠取10只处死,观察腹腔内肿瘤结节数及各脏器转移情况。各组剩余6只裸鼠观察生存时间。结果各组裸鼠致瘤率:对照组10/10例,6mmHg组9/10例,9mmHg组9/10例,12mmHg组9/10例和15mmHg组10/10例,差异无统计学意义。裸鼠腹腔内瘤结节数:6mmHg组(41.70±14.90)与15mmHg组(29.70±9.91)之间差异有统计学意义(P<0.05),其余各组间差异无统计学意义(P>0.05)。各脏器转移情况在各压强组差异无统计学意义(P>0.05)。各组裸鼠生存时间差异无统计学意义(P>0.05)。结论CO2气腹并不增加肿瘤细胞在腹腔内以及对各脏器的侵袭转移能力,对致瘤裸鼠的生存时间无影响。高压强CO2气腹对肿瘤细胞在体内的转移侵袭能力有抑制作用。
Objective To investigate the effects of continuous pneumoperitoneum (CO2) pneumoperitoneum on the invasion and metastasis of colon cancer cells in vitro. Methods The pneumoperitoneum model was established in vitro and the human colon cancer cell line SW1116 was divided into five groups. After being exposed to different pressures of CO2 gas [6, 9, 12, 15 mmHg (1 mmHg = 0.133 kPa)] and conventional culture for 1 h, Into each group of 16 nude mice abdominal cavity (1 × 106 cells / nude mice). After 2 weeks, 10 nude mice in each group were sacrificed and the number of intra-abdominal tumor nodules and the metastasis of each organ were observed. The remaining 6 nude mice in each group were observed survival time. Results The tumorigenic rates of nude mice in each group were 10/10 in the control group, 9/10 cases in the 6 mmHg group, 9/10 cases in the 9 mmHg group, 9/10 cases in the 12 mmHg group and 10/10 cases in the 15 mmHg group. There was no statistically significant difference. The number of intra-abdominal tumor nodules in nude mice: there was a significant difference between 6mmHg group (41.70 ± 14.90) and 15mmHg group (29.70 ± 9.91) (P <0.05), but there was no significant difference between other groups ). There was no significant difference in the metastasis of various organs between pressure groups (P> 0.05). The survival time of nude mice in each group had no significant difference (P> 0.05). Conclusion CO2 pneumoperitoneum does not increase the invasion and metastasis of tumor cells in abdominal cavity and various organs, and has no effect on the survival time of tumor-bearing nude mice. High-pressure CO2 pneumoperitoneum can inhibit the metastasis and invasion of tumor cells in vivo.