采用高效毛细管电泳 ,以羧甲基 -β-环糊精、羟丙基 -β-环糊精、二甲基 -β-环糊精作为手性添加剂对氯霉素前体和甲砜霉素前体对映体进行了分离 .并研究了手性选择剂的类型和浓度、背景电解质的酸度、背景电解质的类型、操作电压和温度等因素对对映体分离的影响 .分离结果表明 ,不仅为此类药物提供了简便、快速的分离分析方法 ,而且还获得了比以往报道更高的分离度 . Chloramphenicol precursors and thiamphenicol were synthesized by high performance capillary electrophoresis with carboxymethyl-β-cyclodextrin, hydroxypropyl-β-cyclodextrin and dimethyl-β-cyclodextrin as chiral additives The enantiomers were separated and the effects of the type and concentration of chiral selectors, the acidity of the background electrolyte, the type of background electrolyte, the operating voltage and the temperature on the enantiomeric separation were studied.The separation results showed that not only For these drugs provides a simple, rapid method of separation and analysis, but also received a higher resolution than previously reported.