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测定了12个不同地理种群的茶树害虫假眼小绿叶蝉Empoasca vitis(Gothe)和1个外群共103个个体的线粒体DNA 16S rRNA基因部分序列,比较其同源性,计算核苷酸组成,并构建单倍型进化关系图。结果表明:在获得的假眼小绿叶蝉536 bp的序列中A+T含量占76.8%,其中56个核苷酸位点存在变异;100个个体共检测出56种单倍型,单倍型多样性指数(H)为0.971,核苷酸多样性指数(Pi)0.006;12个种群间的平均基因流(Nm)为1.64。总群体的固定系数Fst为0.026;AMOVA分子方差分析结果表明假眼小绿叶蝉的遗传分化主要来自于种群内部(97.4%)。各地理种群的遗传距离与地理分布不具有直接对应的关系。根据构建的单倍型进化关系网,各地理种群的单倍型呈现一种混杂的分布格局,未显示出与地理分布的一致性。
The mitochondrial DNA 16S rRNA gene partial sequences of Empoasca vitis (Gothe) and one outgroup of 103 individuals from 12 different geographical populations were determined. The nucleotide sequences, And construct haplogroup evolution relation diagram. The results showed that A + T accounted for 76.8% of the 536 bp sequence of the obtained green leafhopper, 56 of which had 56 nucleotide variations; 56 haplotypes and haplotypes were detected in 100 individuals The diversity index (H) was 0.971, and the nucleotide diversity index (Pi) was 0.006. The average gene flow (Nm) among the 12 populations was 1.64. The Fst of the total population was 0.026. The analysis of variance by AMOVA indicated that the genetic differentiation of the leafhoppers was mainly from the inner part of the population (97.4%). Geographical populations of the genetic distance and geographical distribution does not have a direct correspondence. According to the constructed haplogroup evolution relation network, the haplotypes of each geographical population presented a mixed distribution pattern, which did not show the consistency with the geographical distribution.